HEPES, endotoxin and cell culture tested [CAS# 7365-45-9]
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Catalog numberH006-1KG
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PricePlease ask
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Size1 kg
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AvailabilityPlease contact us to check current availability
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Long descriptionHEPES, endotoxin and cell culture tested [CAS# 7365-45-9]; MW 238.3
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Product formatPowder
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Product categoryBiochemicals
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Product subcategoryBuffers and Supplements
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Shipping methodAmbient temperature
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Storage methodStore at 2˚ to 8˚C.
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DescriptionThe CAS or CASRN registry number for chemical substances and identifying chemicals by the Chemical Abstract Service is a worldwide used identification of chemicals. This chemical can be supplied in purities of 98% or 95% purity by caissonlab. Bulk in kg or liter volumes on request. For cells, cell lines and tissues in culture till half confluency.
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Gene target
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Gene symbolIGHV1-45, SNORD115-45, IGLV5-45, RNU6-9, PIRC45, PIRC47, PIRC38
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Short nameHEPES
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Techniqueculture
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Alternative nameHEPES, endotoxin and cellular culture tested [CAS# 7365-45-9]
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 1-45
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-45
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Synonyms
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Locus
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Discovery year2011-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 5-45
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 9
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 45
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 47
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 38
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
- E05.599.495