HEPES, cell culture tested - 500g
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Catalog number
PM-B2093/500
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Price
Please ask
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Size
2X500g
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Description
HEPES, cell culture tested - 500g is a white free-flowing crystalline powder with the molecular formula C8H18N2O4S and that is used in cell culture media.
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Shipping conditions
-20°C or dry ice
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Storage
Room Temperature
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Shelflife
36 months
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State
Lyophilized
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Chemical description
Miscellaneous products
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Appearance
Physical state at 20 ºC: Solid ; Appearance: Crystalline ; Colour: White with coloured particles
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Density at 20 ºC
1440 kg/m³
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Relative density at 20 ºC
1.44
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PH
NA
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Osmolality
NA
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Endotoxin
NA
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Composition
NA
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Sterility
Non sterile
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Personal precautions
Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
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Uses
Used in biotechnology. For professional user/industrial user only.
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Ordering
To order HEPES, cell culture tested - 500g, please use the Cat. Nr.PM-B2093/500 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for HEPES, cell culture tested - 500g
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
MeSH Data
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Name
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Concept
Scope note:
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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