Heart Infusion Certified

Description

Heart Infusion Certified is an additionaly culture media product and plays a role of enrichment , used also in the propagation of cell line
Other product
Appearance

Light yellow to brownish yellow homogenous free flowing powder,having characteristic odour but not putrescent.
Solubility

Soluble in distilled/ purified water, insoluble in alcohol and ether.
Clarity

1% w/v aqueous solution is clear without any haziness and free from visible foreign/black particles after autoclaving at 15 lbs pressure (121ºC) for 15 minutes.
Reaction

Reaction of 2% w/v aqueous solution at 25ºC.
PH

6.00- 7.00
Total aerobic microbial count cfu gm

By plate method when incubated at 30-35°C for not less than 3 days. Bacterial Count :
Total Yeast and mould count cfu gm

By plate method when incubated at 20-25°C for not less than 5 days. Yeast & mould Count :
Test for Pathogens

1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosaNegative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of sample6. Clostridia- Negative in 10 gms of sample
Indole Test

Tryptophan content: Passes
Cultural response

Cultural response observed after an incubation at 35-37ºC for 18-24 hours by preparing BHI Agar (M211) using HI Certified as an ingredient.
Chemical Analysis

TotalNitrogen >= 10.0% AminoNitrogen >= 3.80% Sodium chloride
Storage

Store below 30°C.