HDAC8 Inhibitor Screening Kit (Fluorometric)
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Catalog number
K368-100
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Price
Please ask
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Size
100 assays
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Description
Simple Assay, HTS
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Summary
• Detection method- Fluorescence (Em/Ex = 380/500 nm) • Applications- Suitable for high throughput screening of HDAC-8 inhibitors
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Sample Type
Testing candidate HDAC8 inhibitors
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Features Benefits
• Simple procedure; takes ~ 60 min • Fast and convenient • Kit contains the necessary reagents for testing putative HDAC-8 inhibitors
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-8 Inhibitor screening Kit, HDAC-8 deacetylates the substrate (R-H-K(Ac)-K(Ac)-AFC) followed by an enzymatic cleavage to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. In the presence of an HDAC-8 inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release AFC. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-8 inhibitors. Trichostatin A is included as a control inhibitor to compare the efficacy of the test inhibitors.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbol
HDAC8
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Short name
HDAC8 Inhibitor Screening Kit (Fluorometric)
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Alternative name
HDAC8 suppressor detection reagent (fluorescence)
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 8
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Synonyms gene
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Synonyms gene name
- histone deacetylase-like 1
- Wilson-Turner X-linked mental retardation syndrome
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histone deacetylases, class I
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VEGA ID
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Locus Specific Databases
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