HDAC3 Immunoprecipitation (IP) & Activity Assay Kit
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Catalog number
K344-25
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Price
Please ask
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Size
25 assays
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Description
Only kit on the market for both IP and measuring activity of HDAC3
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Summary
• Detection method - Fluorescence (Ex/Em=368/442 nm) • Applications: - Immunoprecipitation of HDAC3 complex from cell & nuclear extract(s) - Measurement of HDAC3 activity of immunoprecipitated complex/purified enzyme - Screening for activators or inhibitors of HDAC3
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Sample Type
Human, Mouse or Rat • Cell lysate and Nuclear Extract
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Features Benefits
• Simple, fast and convenient. • Only kit on the market for both IP as well as measuring activity of HDAC3.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. These proteins have crucial roles in development and physiology, especially in the heart and nervous system. They are also deeply involved in cellular proliferation, cell cycle and apoptosis. HDAC3 is primarily localized in the nucleus, but can also be found in the cytoplasm and at the plasma membrane. The HDAC3 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC3 complex from cells & tissues and to measure HDAC3 activity fluorometrically. HDA3 is immunoprecipitated from cell or nuclear extract(s) using HDAC3 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
HDAC3
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Short name
HDAC3 Immunoprecipitation (IP) & Activity Assay Kit
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Technique
Immunoprecipitation, Assay, IP, assays, Immunoprecipitation
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Host
Assay
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Alternative name
histone deacetylase 3 Immunoprecipitation (IP) & Activity test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 3
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-01-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NCoR/SMRT transcriptional repression complex subunits
- Histone deacetylases, class I
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VEGA ID
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