HDAC1 (C-Terminus HIS-FLAG tag) - Active Enzyme
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Catalog numberX1737E
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PricePlease ask
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Size20 μg
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CategoryProteins & Peptides
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Long descriptionAcetylation of the tail of histone is known to cause chromatin to adopt a more ÔopenÕ 3D conformation, allowing trans factors grerater access to DNA. Histone acetyltransferases (HATs), complexes interact with sequence-specific activator proteins and target specific genes. In addition to histones, HATs acetylate non-histone proteins, implicating them in a wide variety of regulartory roles for these enzymes. By comparison, histone deacetylation promotes a more ÔclosedÕ chromatin conformation and in general leads to repression of gene activity. Mammalian histone deacetylases are divided into three classes on the basis of their similarity to various the yeast deacetylases. Class I (HDACs 1, 2, 3 and 8) related to the yeast Rpd3-like proteins, class II (HDACs 4, 5, 6, 7, 9 and 10) related to yeast Hda1-like proteins and class III related to the yeast protein Sir2. Inhibitors of HDAC have enormous potential as cancer therapeutic agents.
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Antibody come fromHybridoma produced by the fusion of splenocytes from Lewis rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface and mouse Sp2/0 myeloma cells.
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Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide.
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Clonenot specified
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Antigen antibody binding interactionHDAC1 (C-Terminus HIS-FLAG tag) - Active Enzyme
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Antibody is raised inSf9 cells
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Antibody s reacts withHuman
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationUseful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.
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Antibody s suited forAntibody suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on frozen sections and flow cytometry (1:200 dilution). Optimal concentration should be evaluated by serial dilutions.
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Storage-20ºC
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Relevant references1. Hallmann, R., et al. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9._x000B__x000B_2. Hammel, M., et al. (2001). Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74._x000B_
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionEnzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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PropertiesAn anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag, histidine
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Gene target
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Gene symbolHAL, HDAC1, VIS1, AKR1B10, TRH-GTG1-1, TRH-GTG1-3, TRH-GTG2-1, TRH-GTG3-1, TRH-GTG1-2, TRH-GTG1-4
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Short nameHDAC1 ( HIS- tag) - Active Enzyme
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TechniqueEnzyme, antibodies directed against c-terminus peptides can be suited for IHC because in the quaternary structure the c-terminus of this protein is often at the outside of the cell membrane. enzymes, Tags
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Isotypenot specified
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Labelunlabeled
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Alternative namehistone deacetylase 1 (C-Terminus histidine-FLAG detection labelled) - functionnal Enzyme
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Alternative techniqueenzymes
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Alternative to gene targethistone deacetylase 1, this GO N-10 and HD1 and RPD3 and RPD3L1, HDAC1 and IDBG-95712 and ENSG00000116478 and 3065, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, HDAC1 and IDBG-644586 and ENSBTAG00000012698 and 404126
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Gene info
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Identity
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Gene
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Long gene namehistidine ammonia-lyase
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Synonyms gene
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Locus
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Discovery year1988-08-31
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namehistone deacetylase 1
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NuRD complex subunits
- Histone deacetylases, class I
- MiDAC complex subunits
- EMSY complex
- SIN3 histone deacetylase complex subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameviral integration site 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-11-25
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namealdo-keto reductase family 1 member B10
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Synonyms gene
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Synonyms gene name
- aldo-keto reductase family 1, member B10 (aldose reductase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-08-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Aldo-keto reductases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 4 (anticodon GUG)
- transfer RNA-His (GTG) 1-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-21
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 9 (anticodon GUG)
- transfer RNA-His (GTG) 1-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (GTG) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 11 (anticodon GUG)
- transfer RNA-His (GTG) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Low confidence cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 8 (anticodon GUG)
- transfer RNA-His (GTG) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-2
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 10 (anticodon GUG)
- transfer RNA-His (GTG) 1-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 7 (anticodon GUG)
- transfer RNA-His (GTG) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs