HDAC1 (C-Terminus HIS-FLAG tag) - Active Enzyme

  • Catalog number
    X1737E
  • Price
    Please ask
  • Size
    20 μg
  • Category
    Proteins & Peptides
  • Long description
    Acetylation of the tail of histone is known to cause chromatin to adopt a more ÔopenÕ 3D conformation, allowing trans factors grerater access to DNA. Histone acetyltransferases (HATs), complexes interact with sequence-specific activator proteins and target specific genes. In addition to histones, HATs acetylate non-histone proteins, implicating them in a wide variety of regulartory roles for these enzymes. By comparison, histone deacetylation promotes a more ÔclosedÕ chromatin conformation and in general leads to repression of gene activity. Mammalian histone deacetylases are divided into three classes on the basis of their similarity to various the yeast deacetylases. Class I (HDACs 1, 2, 3 and 8) related to the yeast Rpd3-like proteins, class II (HDACs 4, 5, 6, 7, 9 and 10) related to yeast Hda1-like proteins and class III related to the yeast protein Sir2. Inhibitors of HDAC have enormous potential as cancer therapeutic agents.
  • Antibody come from
    Hybridoma produced by the fusion of splenocytes from Lewis rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface and mouse Sp2/0 myeloma cells.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide.
  • Clone
    not specified
  • Antigen antibody binding interaction
    HDAC1 (C-Terminus HIS-FLAG tag) - Active Enzyme
  • Antibody is raised in
    Sf9 cells
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.
  • Antibody s suited for
    Antibody suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on frozen sections and flow cytometry (1:200 dilution). Optimal concentration should be evaluated by serial dilutions.
  • Storage
    -20ºC
  • Relevant references
    1. Hallmann, R., et al. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9._x000B__x000B_2. Hammel, M., et al. (2001). Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74._x000B_
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
  • Properties
    An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Conjugation
    Flag, histidine
  • Gene target
    HDAC1   HIS   tag   Active  
  • Gene symbol
    HAL, HDAC1, VIS1, AKR1B10, TRH-GTG1-1, TRH-GTG1-3, TRH-GTG2-1, TRH-GTG3-1, TRH-GTG1-2, TRH-GTG1-4
  • Short name
    HDAC1 ( HIS- tag) - Active Enzyme
  • Technique
    Enzyme, antibodies directed against c-terminus peptides can be suited for IHC because in the quaternary structure the c-terminus of this protein is often at the outside of the cell membrane. enzymes, Tags
  • Isotype
    not specified
  • Label
    unlabeled
  • Alternative name
    histone deacetylase 1 (C-Terminus histidine-FLAG detection labelled) - functionnal Enzyme
  • Alternative technique
    enzymes
  • Alternative to gene target
    histone deacetylase 1, this GO N-10 and HD1 and RPD3 and RPD3L1, HDAC1 and IDBG-95712 and ENSG00000116478 and 3065, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, HDAC1 and IDBG-644586 and ENSBTAG00000012698 and 404126
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 4 (anticodon GUG)
    • transfer RNA-His (GTG) 1-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-21
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-3
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 9 (anticodon GUG)
    • transfer RNA-His (GTG) 1-3
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (GTG) 2-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 11 (anticodon GUG)
    • transfer RNA-His (GTG) 2-1
  • Synonyms
  • GenBank acession
  • Locus
    1
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Low confidence cytoplasmic transfer RNAs
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-2
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 10 (anticodon GUG)
    • transfer RNA-His (GTG) 1-2
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-4
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 7 (anticodon GUG)
    • transfer RNA-His (GTG) 1-4
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Similar products
Filters
Contact
Chat with gentaur.com employee