HDAC Activity Colorimetric Assay Kit
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Catalog number
K331-100
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Price
Please ask
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Size
100 assays
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Description
Two step assay, HTS
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Summary
• Detection method- Absorbance (400 or 405 nm) • Species reactivity- Mammalian • Applications- Well suited for high throughput applications. HDAC inhibitors and substrates and related antibodies are also available separately.
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Sample Type
Cell lysate, nuclear extract
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Features Benefits
• Simple two-step procedure; takes around than 1 hour • Fast and convenient • The assay method eliminates radioactivity/extractions/ and/or chromatography as used in the traditional assays.
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Storage Conditions
-80°C
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Shipping Conditions
dry ice
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Shelf life
12 months
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Background
Inhibition of histone deacetylases (HDACs) has been implicated to modulate transcription and to induce apoptosis or differentiation in cancer cells. However, screening HDAC inhibitory compounds has proven to be difficult over the past due to the lack of convenient tools for analyzing HDAC activity. The new Colorimetric HDAC Activity Assay Kit provides a fast and convenient colorimetric method that eliminates radioactivity, extractions, or chromatography, as used in the traditional assays. The new method requires only two easy steps, both performed on the same microtiter plate. First, the HDAC colorimetric substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g., HeLa nuclear extract or your own samples). Deacetylation of the substrate sensitizes the substrate, so that, in the second step, treatment with the Lysine Developer produces a chromophore. The chromophore can be easily analyzed using an ELISA plate reader or spectrophotometer. The assay is well suited for high throughput screening applications. HDAC inhibitors and antibodies are also available separately.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Gene symbol
HDAC4, HDAC9
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Short name
HDAC Activity Colorimetric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
HDAC Activity colorimetry test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 4
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Synonyms gene
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Synonyms gene name
- brachydactyly-mental retardation syndrome
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histone deacetylases, class IIA
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 9
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Synonyms
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GenBank acession
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Locus
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Discovery year
2002-09-06
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Entrez gene record
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Pubmed identfication
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Classification
- Histone deacetylases, class IIA
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VEGA ID
Product images
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