HDAC-8, human recombinant

• Catalog number
  7618-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  HDAC-8,HDACL1, Histone deacetylase 8
• Alternative_names
  HDAC-8,HDACL1, Histone deacetylase 8
• Description
  Critical regulator of transcription, cell cycle progression, and developmental events
• Recombinant
  Yes
• Source
  Sf9 insect cells
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Activity of rh-HDAC8 was tested using Arg-His-Lys(Ac)-Lys(Ac)-AFC, a fluorogenic, acetylated peptide based on residues 379-382 of p53 as a substrate.
• Unit Definition
  One unit of the recombinant HDAC8 is defined as the amount of enzyme that deacetylates 1 pmol of substrate Arg-His-Lys(Ac)-Lys(Ac)-AFC per minute at 37°C in a reaction solution containing 50 mM Tris, pH 7.5, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2 and 1 mg/ml BSA.
• Molecular Weight
  44 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  Liquid in 25 mM Tris, pH 7.5, 100 mM NaCl, 2.7 mM KCl, 3 mM MgCl2, 10 % glycerol.
• Background Information
  Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases. HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation. Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor Trichostatin A (TSA).
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  HDAC-8  
• Gene symbol
  HDAC4, SNORD114-8, RNU6-8, SNORD113-8, HDAC9, SNORD116-8, IGKV2OR2-8, HDAC8, MIR1302-8, PIRC35
• Short name
  HDAC-8, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  HDAC-8, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:14063
• Gene
  HDAC4
• Long gene name
  histone deacetylase 4
• Synonyms gene
  • BDMR
• Synonyms gene name
  • brachydactyly-mental retardation syndrome
• Synonyms
  • KIAA0288
  • HDAC-A
  • HDACA
  • HD4
  • HA6116
  • HDAC-4
• GenBank acession
  • AB006626
• Locus
  2q37.3
• Discovery year
  2000-11-28
• Entrez gene record
  9759
• Pubmed identfication
  • 10206986
  • 10220385
  • 20691407
• RefSeq identity
  • NM_006037
• Classification
  • Histone deacetylases, class IIA
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000133344

Gene info

• Identity
  HGNC:32996
• Gene
  SNORD114-8
• Long gene name
  small nucleolar RNA, C/D box 114-8
• Synonyms
  • 14q(II-8)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767584
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003200.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:34285
• Gene
  RNU6-8
• Long gene name
  RNA, U6 small nuclear 8
• Synonyms
  • U6-8
• Locus
  14q12
• Discovery year
  2008-06-10
• Entrez gene record
  101954278
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_104088
• Classification
  • Small nuclear RNAs

Gene info

• Identity
  HGNC:32987
• Gene
  SNORD113-8
• Long gene name
  small nucleolar RNA, C/D box 113-8
• Synonyms
  • 14q(I-8)
• Locus
  14q32.31
• Discovery year
  2006-09-20
• Entrez gene record
  767568
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003236.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:14065
• Gene
  HDAC9
• Long gene name
  histone deacetylase 9
• Synonyms
  • KIAA0744
  • HDAC
  • MITR
  • HD7
  • HDAC7B
• GenBank acession
  • AF124924
• Locus
  7p21.1
• Discovery year
  2002-09-06
• Entrez gene record
  9734
• Pubmed identfication
  • 10523670
  • 10487760
• Classification
  • Histone deacetylases, class IIA
• VEGA ID
  OTTHUMG00000152487

Gene info

• Identity
  HGNC:33074
• Gene
  SNORD116-8
• Long gene name
  small nucleolar RNA, C/D box 116-8
• Synonyms
  • HBII-85-8
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033420
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003323
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5811
• Gene
  IGKV2OR2-8
• Long gene name
  immunoglobulin kappa variable 2/OR2-8 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2/OR2-8
  • immunoglobulin kappa variable 2/OR2-8 pseudogene
• Synonyms
  • IGKV2/OR2-8
• GenBank acession
  • X51880
• Locus
  2q11.2
• Discovery year
  2000-04-18
• Entrez gene record
  100996600
• RefSeq identity
  • NG_011662
• Classification
  • Immunoglobulin kappa (IGK) orphons

Gene info

• Identity
  HGNC:13315
• Gene
  HDAC8
• Long gene name
  histone deacetylase 8
• Synonyms gene
  • HDACL1
  • WTS
  • MRXS6
• Synonyms gene name
  • histone deacetylase-like 1
  • Wilson-Turner X-linked mental retardation syndrome
• Synonyms
  • RPD3
  • KDAC8
• GenBank acession
  • AF230097
• Locus
  Xq13.1
• Discovery year
  2000-11-28
• Entrez gene record
  55869
• Pubmed identfication
  • 10756090
  • 10922473
  • 22889856
• RefSeq identity
  • NM_018486
• Classification
  • Histone deacetylases, class I
• VEGA ID
  OTTHUMG00000021814
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:35300
• Gene
  MIR1302-8
• Long gene name
  microRNA 1302-8
• Synonyms gene
  • MIRN1302-8
• Synonyms
  • hsa-mir-1302-8
• Locus
  9q22.33
• Discovery year
  2008-11-03
• Entrez gene record
  100302223
• RefSeq identity
  • NR_031637
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:37539
• Gene
  PIRC35
• Long gene name
  piwi-interacting RNA cluster 35
• Locus
  8
• Discovery year
  2009-11-05
• Entrez gene record
  100313855
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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