Recombinant Human Tryptase epsilon/Brain-Specific Serine Protease 4/BSSP-4 (C-6His)

• Catalog number
  C383-500
• Price
  Please ask
• Size
  500 ug

• Description
  Recombinant Human Tryptase epsilon is produced by our Mammalian expression system and the target gene encoding Ala33-Ser317 is expressed with a 6His tag at the C-terminus.
• Species reactivity
  Human
• Origin
  Human cells
• Peptide sequence
  ARIPVPPACGKPQQLNRVVGGEDSTDSEWPWIVSIQKNGTHHCAGSLLTSRWVITAAHCFKDNLNKPYLFSVLLGAWQLGNPGSRSQKVGVAWVEPHPVYSWKEGACADIALVRLERSIQFSERVLPICLPDASIHLPPNTHCWISGWGSIQDGVPLPHPQTLQKLKVPIIDSEVCSHLYWRGAGQGPITEDMLCAGYLEGERDACLGDSGGPLMCQVDGAWLLAGIISWGEGCAERNRPGVYISLSAHRSWVEKIVQGVQLRGRAQGGGALRAPSQGSGAAARSHHHHHH
• Estimated molecular weight
  31,56 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Dry ice/ice packs
• Package form
  Supplied as a 0.2 µm filtered solution of 20mM HAc-NaAc, 150mM NaCl, 10% Glycerol, pH 4.5.
• Storage conditions
  Store at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• Reconstitution conditions
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• UniProt number
  Q9GZN4
• Other related products
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants
• Additional description
  Serine protease, D- or L-serine arginine rich enzyme of serine threonine kinase with serine that is encoded by the codons UCU, UCC, UCA, UCG, AGU and AGC is an ɑ-amino acid that is used in the biosynthesis of proteins. It contains an α-amino group (which is in the protonated −NH+ 3 form under biological conditions), a carboxyl group. It is non-essential in humans, meaning the body can synthesize it.

• Gene target
  Tryptase   epsilon/Brain-Specific   Serine   Protease   4/BSSP-4   C-6His  
• Gene symbol
  PRSS22, PRSS12, KLK6, MIR1302-4, PIRC18, PIRC16, SNORD114-4, IGKV2OR2-4
• Short name
  Recombinant Tryptase epsilon/Brain-Specific Serine Protease 4/BSSP-4 (C-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Human Tryptase epsilon/BSSP-4(C-6His)
• Alternative technique
  rec
• Tissue
  brain

Gene info

• Identity
  HGNC:14368
• Gene
  PRSS22
• Long gene name
  serine protease 22
• Synonyms gene name
  • protease, serine 22
• Synonyms
  • hBSSP-4
  • BSSP-4
  • SP001LA
• Synonyms name
  • brain-specific serine protease 4
• GenBank acession
  • AF321182
• Locus
  16p13.3
• Discovery year
  2001-09-26
• Entrez gene record
  64063
• Pubmed identfication
  • 11602603
  • 15701722
• RefSeq identity
  • NM_022119
• Classification
  • Serine proteases
• VEGA ID
  OTTHUMG00000128961

Gene info

• Identity
  HGNC:9477
• Gene
  PRSS12
• Long gene name
  serine protease 12
• Synonyms gene name
  • protease, serine 12
• Synonyms
  • BSSP-3
  • MRT1
• Synonyms name
  • neurotrypsin
  • motopsin
  • brain-specific serine protease 3
  • mental retardation, autosomal recessive 1
• GenBank acession
  • AJ001531
• Locus
  4q26
• Discovery year
  1998-10-02
• Entrez gene record
  8492
• Pubmed identfication
  • 9540828
  • 9245503
• Classification
  • Scavenger receptor cysteine rich domain containing
  • Serine proteases
• VEGA ID
  OTTHUMG00000161166

Gene info

• Identity
  HGNC:6367
• Gene
  KLK6
• Long gene name
  kallikrein related peptidase 6
• Synonyms gene
  • PRSS9
  • PRSS18
• Synonyms gene name
  • protease, serine, 18
  • kallikrein 6 (neurosin, zyme)
• Synonyms
  • Bssp
  • Klk7
  • neurosin
• GenBank acession
  • U62801
• Locus
  19q13.41
• Discovery year
  1997-06-09
• Entrez gene record
  5653
• Pubmed identfication
  • 9003450
  • 16800724
  • 16800723
• RefSeq identity
  • NM_002774
• Classification
  • Kallikreins
  • Serine proteases
• VEGA ID
  OTTHUMG00000183098

Gene info

• Identity
  HGNC:35296
• Gene
  MIR1302-4
• Long gene name
  microRNA 1302-4
• Synonyms gene
  • MIRN1302-4
• Synonyms
  • hsa-mir-1302-4
• Locus
  2q33.3
• Discovery year
  2008-11-03
• Entrez gene record
  100302130
• RefSeq identity
  • NR_031633
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:37522
• Gene
  PIRC18
• Long gene name
  piwi-interacting RNA cluster 18
• Locus
  4
• Discovery year
  2009-11-05
• Entrez gene record
  100313906
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37520
• Gene
  PIRC16
• Long gene name
  piwi-interacting RNA cluster 16
• Locus
  4
• Discovery year
  2009-11-05
• Entrez gene record
  100313849
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:32992
• Gene
  SNORD114-4
• Long gene name
  small nucleolar RNA, C/D box 114-4
• Synonyms
  • 14q(II-4)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767580
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003196.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5809
• Gene
  IGKV2OR2-4
• Long gene name
  immunoglobulin kappa variable 2/OR2-4 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 2/OR2-4
  • immunoglobulin kappa variable 2/OR2-4 pseudogene
• Synonyms
  • IGKV2/OR2-4
• GenBank acession
  • X51883
• Locus
  2q11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28858
• Pubmed identfication
  • 2111145
• Classification
  • Immunoglobulin kappa (IGK) orphons

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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