Hamster IgG, pooled Armenian/Syrian, Negative Control, Clone: 530-6, RPE; flow
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Catalog numberOBT0423R
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PricePlease ask
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Size0.1 mg
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Alternative name 1Hamster IgG, pooled Armenian/Syrian, Negative Control, Clone: 530-6, RPE; flow
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Alternative name 2Hamster IgG, pooled Armenian/Syrian, Negative Control, Clone: 530-6, RPE; flow
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Alternative name 3Hamster IgG, pooled Armenian/Syrian, Negative Control, Clone: 530-6, RPE; flow
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Alternative name 4anti- IgG pooled Armenian/Syrian Negative Control Clone: 530-6 flow
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Alternative name 5IgG pooled Armenian/Syrian Negative Control Clone: 530-6 flow
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Additional descriptionIsotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
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PropertiesArmenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Gene symbolRPE, MIR1302-6, PIRC24, PIRC28, PIRC26, ADCP1, PIRC27
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Short nameIgG, pooled Armenian/Syrian, Negative Control, Clone: 530-6, RPE;
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TechniqueNegative, Control, Flow, IgG, controls, IgGs, negatif
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HostHamster
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IsotypeIgG, IgG
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Labelrpe
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SpeciesHamster, Hamsters
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Alternative nameHamster Immunoglobulin G, pooled Armenian/Syrian, Negative reference, clonality: 530-6, RPE; flow
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Alternative techniquecontrols, immunoglobulins
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Tissuecontrol
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Gene info
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Identity
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Gene
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Long gene nameribulose-5-phosphate-3-epimerase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-6
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 24
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 28
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 26
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameadenosine deaminase complexing protein 1
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 27
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
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Tree numbers
- E01.370.225.812.735.830
- E05.200.812.735.830
- E05.478.566.380.810
- E05.478.566.639.810
- E05.478.594.760.830
- E05.601.470.380.810
- E05.601.470.639.810
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data