HAMSTER ANTI MOUSE BIM:FITC Antibody
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Catalog number
GEN224424
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Price
Please ask
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Size
100ug
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Also known as
BIM
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Other names
bcl-2-like protein 11 isoform 3; Bcl-2-like protein 11; bcl-2-like protein 11; bcl2-L-11; Bcl2 interacting mediator of cell death; bcl2-interacting mediator of cell death; BCL2-like 11 (apoptosis facilitator); Bcl2-interacting mediator of cell death
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
BIM
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Gene name synonims
N/A
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Other gene names
Bcl2l11; Bcl2l11; Bim; Bod; 1500006F24Rik; Bim; Bcl2-L-11
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG
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Clone
151-149
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Host organism
Hamster
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
BIM This item is specific for human Bcl-2 interacting mediator of cell death (Bim), also known as Bod. Bim is a pro-apoptotic protein within the Bcl-2 family, which functions within the intrinsic apoptosis pathway. Bim is known to interact with pro-survival proteins including Bcl-2, Bcl-XL and Bcl-w._x000D__x000D_It is expressed in a variety of cell types, where it is maintained in an inactive conformation by phosphorylation or through binding to the microtubule-associated dynein motor complex. Upon certain apoptotic stimuli, Bim is released from microtubules and mediates caspase-dependent apoptosis._x000D_; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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Concentration
IgG concentration 0.1 miligram per 1 mililiter
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. the antibody is photosensitive and should be protected from light. Shelf Life: 18 months from date of dispatch.
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Tested applications
Flow Cytometry (FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
BCL2L11
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Short name
ANTI MOUSE BIM:FITC Antibody
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Technique
anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
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Host
Hamster
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Label
FITC
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Species
Mouse, Hamsters, Mouses
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Alternative name
Anti-HAMSTER ANTI MOUSE BIM:FITC
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Alternative technique
antibodies, murine, fluorescine
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Gene info
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Identity
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Gene
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Long gene name
BCL2 like 11
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Synonyms gene name
- BCL2-like 11 (apoptosis facilitator)
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-12-10
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Entrez gene record
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Pubmed identfication
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Classification
- BCL2 homology region 3 (BH3) only
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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