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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
chromatin immunoprecipiation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), immunofluorescence (IF), peptide array (PA), western blot (WB)
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Connected products
AS16 3190-10 | anti-H3K4me3 | histone H3, trimethylated lysine 4 (H3K4me3) (10 µg), rabbit antibodies AS16 3187 | anti-H3K56me1 | Histone H3 (monomethyl Lys56), polyclonal antibodies AS16 3182 | anti-H3K18me2 | Histone H3 (dimethyl Lys18), rabbit antibodies AS16 3183 | anti-H3K18me3 | Histone H3 (trimethyl Lys18), rabbit antibodies AS16 3181 | anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodiesAS16 3197 | anti-anti-H3core, monoclonal antibodiesAS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710collection of antibodies to epigenetics
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Recommended dilutions for use
0.5-1 µg/IP (ChIP/ChIP-seq), 1: 20 000 (Dot), 1: 1000 (ELISA), 1: 500 (IF), 1: 20 000 (PA), 1: 1000 (WB)
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Molecular weight expected аpparent
15 kDa
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Verified reactivity
human
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Possible reactivity
A.thaliana, C. elegans, mouse, Populus sp. , S. lycopersicum, Z. mays
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No reactivity
No confirmed exceptions from predicted reactivity known at the moment
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Supplementary information
To be added when available
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References
To be added when available, antibody released in February 2016.
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Scientific context
Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K27 is associated with inactive genomic regions.
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Notes
Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
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Protein number
P68431
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TAIR number
Refer to NCBI
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