H1, histone H1

• Catalog number
  AS11 1801
• Price
  Please ask
• Size
  50 µg

• Available ordering format
  Lyophilized
• Immunogen
  native H1 protein purified from Nicotiana tabaccum (H1A, H1B H1C,D,E,F)
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), immunocytochemistry (ICC)
• Connected products
  AS10 710 | H3 | histone H3, rabbit antibody AS11 1753 | HDT3 | histone deacetylase, rabbit antibody collection of antibodies to DNA/RNA/cell cyclePlant protein extraction buffer Secondary antibodies
• Recommended dilutions for use
  1 : 5000 with standard ECL (WB), 1: 100 - 1: 500 (ICC)
• Molecular weight expected аpparent
  15 | 17 kDa
• Verified reactivity
  Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum
• Possible reactivity
  Lathyrus sativus, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Vicia faba
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Protocol for isolation of cytosolic and nuclear fractions can be found here.Protocol for immunostatining in whole-mount plant tissues: An efficient method for quantitative, single-cell analysis of chromatin modification and nuclear architecture in whole-mount ovules in Arabidopsis Wenjing She, Daniel Grimanelli, Célia Baroux Journal of Vizualized Experiments (JoVE), in press
• References
  She and Baroux (2015). Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. | doi: 10.3389/fpls.2015.00294. She et al. (2013). Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140(19):4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)
• Scientific context
  Histone 1 (H1) is a protein located in nuclei, incorporated into chromatin.
• Notes
  None
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  histone  
• Gene symbol
  H1-0, H1-10, H1-9P
• Short name
  H1, histone H1
• Alternative name
  H1, histone H1

Gene info

• Identity
  HGNC:4714
• Gene
  H1-0
• Long gene name
  H1.0 linker histone
• Synonyms gene
  • H1FV
  • H1F0
• Synonyms gene name
  • H1 histone family, member 0
  • H1 histone family member 0
• Synonyms
  • H1.0
• Synonyms name
  • H1.0, H1(0), H1-0
• GenBank acession
  • X03473
• Locus
  22q13.1
• Discovery year
  1988-07-20
• Entrez gene record
  3005
• Pubmed identfication
  • 3084796
• RefSeq identity
  • NM_005318
• Classification
  • H1 histones
• VEGA ID
  OTTHUMG00000150659

Gene info

• Identity
  HGNC:4722
• Gene
  H1-10
• Long gene name
  H1.10 linker histone
• Synonyms gene
  • H1FX
• Synonyms gene name
  • H1 histone family, member X
  • H1 histone family member X
• Synonyms
  • MGC15959
  • MGC8350
  • H1X
• GenBank acession
  • D64142
• Locus
  3q21.3
• Discovery year
  1999-06-11
• Entrez gene record
  8971
• Pubmed identfication
  • 8964515
  • 9439656
• RefSeq identity
  • NM_006026
• Classification
  • H1 histones
• VEGA ID
  OTTHUMG00000159450

Gene info

• Identity
  HGNC:30616
• Gene
  H1-9P
• Long gene name
  H1.9 linker histone, pseudogene
• Synonyms gene
  • HILS1
  • H1-9
• Synonyms gene name
  • histone linker H1 domain, spermatid-specific 1
  • histone linker H1 domain, spermatid-specific 1 (pseudogene)
  • H1.9 linker histone (pseudogene)
• Synonyms
  • H1.9
• GenBank acession
  • AY286318
• Locus
  17q21.33
• Discovery year
  2005-12-16
• Entrez gene record
  373861
• Pubmed identfication
  • 12920187
  • 30068355
• RefSeq identity
  • NR_024193
• Classification
  • H1 histones
• VEGA ID
  OTTHUMG00000162025

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