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Availability
Please contact us to check the availability
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Immunogen
Purified elastin from human skin
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Specificity
Human Elastin 100%, human Collagen Type I, II, III, IV, V
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Species reactivity
Human, cross-rectivity with rat Elastin < 0.1%
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Product presentation
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
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Product category
Primary Antibody
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Antibody s raised in
Guinea Pig
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Antibody s isotype
IgG
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Antibody s clone
polyclonal
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Product is supplied as
purified, lyophilized
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Recommended concentration for use
IFA ≥ 1:80, IHC(P) ≥ 1:500 - 1:1000, ELISA ≥ 1:200 (OD ≥ 500)
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Protein concentration
app. 1 mg/ml
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Positive control
Human skin or aorta
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Pretreatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
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Tested applications
IFA, IHC(P), ELISA, RIA
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Recommended secondary reagent
Anti-Guinea pig IgG-conjugates
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Storage method
Store at -20°C
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UniProt number
P155Go to UniProt/SwissProt2 (ELN_HUMAN)
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Litterature
1. Demarchez M., Hartmann D.J., Herbage D., Ville G., and Prunieras M. (1987) Wound-healing of human skin transplanted onto the nude mouse. 2. an immunohistochemical and ultrastructural study of the epidermal basment-membrane zone reconstruction and connenc 2. Demarchez M., Hartmann D.J., and Prunieras M. (1987) An immunohistochemical study of the revascularization process in human skin transplanted onto the nude mouse. Transplantation 43; 896-903. 3. Lethias C., Hartmann D.J., Masmejean M., Ravazzola M., Sabbagh I., Ville G., Herbage D., Eloy R. (1987) Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique. J. Histochem. Cytochem. 35, 15-21.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Pigs and the smaller guinea pigs are frequent used as models for humans. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Group
Polyclonals and antibodies
