Guanylate kinase, human recombinant

• Catalog number
  P1101-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  GMK, GMP kinase, Guanylate kinase
• Alternative_names
  GMK, GMP kinase, Guanylate kinase
• Description
  Essential for recycling GMP and indirectly, cGMP.
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH.
• Biological activity
  Specific activity is >100 units/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH
• Results
  >100 units/mg
• Unit Definition
  One unit is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH.
• Molecular Weight
  23.9 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl
• Background Information
  GUK1, also known as GMK, belongs to the guanylate kinase family. This protein exists as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP. Via its catalytic activity, GUK1 is thought to participate in regulating the supply of guanine nucleotides to signal transduction pathways. Overexpression of GUK1 is associated with pituitary adenocarcinomas, suggesting that GUK1 is involved in tumorigenesis. Recombinant human GUK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Guanylate   kinase  
• Gene symbol
  BEGAIN, GUK2, GUK1
• Short name
  Guanylate kinase, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Guanylate phosphorylation catalyst, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:24163
• Gene
  BEGAIN
• Long gene name
  brain enriched guanylate kinase associated
• Synonyms gene name
  • brain-enriched guanylate kinase-associated homolog (rat)
  • brain-enriched guanylate kinase-associated
• Synonyms
  • KIAA1446
• GenBank acession
  • BC002607
• Locus
  14q32.2
• Discovery year
  2007-08-16
• Entrez gene record
  57596
• Pubmed identfication
  • 10819331
• RefSeq identity
  • NM_020836
• VEGA ID
  OTTHUMG00000171589

Gene info

• Identity
  HGNC:4694
• Gene
  GUK2
• Long gene name
  guanylate kinase 2
• Locus
  1q32.1-q42
• Discovery year
  2001-06-22
• Entrez gene record
  2988
• Pubmed identfication
  • 5036631
  • 4377261

Gene info

• Identity
  HGNC:4693
• Gene
  GUK1
• Long gene name
  guanylate kinase 1
• GenBank acession
  • BC006249
• Locus
  1q42.13
• Discovery year
  2001-06-22
• Entrez gene record
  2987
• Pubmed identfication
  • 8647247
• RefSeq identity
  • NM_000858
• VEGA ID
  OTTHUMG00000039503

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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