GSTA4, human recombinant
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Catalog number7800-100
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PricePlease ask
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Size100 ug
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SynonymsGlutathione S-transferase alpha 4, GSTA4-4, GTA4
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Alternative_namesGlutathione S-transferase alpha 4, GSTA4-4, GTA4
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DescriptionInvolved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds
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RecombinantYes
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SourceE. coli.
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activitySpecific activity > 0.9 units/mg, one unit is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2, 4- dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25ºC.
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ResultsSpecific activity > 0.9 units/mg
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Unit DefinitionOne unit is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2, 4- dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25ºC.
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Molecular Weight28.3 kDa (246 aa, 1-222 aa + His Tag)
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form description1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol, 2 mM DTT and 100 mM NaCl.
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Background InformationGSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.
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Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolGSTA4
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Short nameGSTA4, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameGSTA4, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameglutathione S-transferase alpha 4
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Synonyms gene name
- glutathione S-transferase A4
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GenBank acession
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Locus
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Discovery year1998-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Soluble glutathione S-transferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data