GST-Δ/GSTD2, Active Recombinant (Bombyx mori (Silk moth))

• Catalog number
  7840-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Glutathione S-Transferase Delta, GSTd2
• Alternative_names
  Glutathione S-Transferase Delta, GSTd2
• Description
  ≥98% Pure Bombyx mori recombinant GST-Δ/GSTD2, that plays an important role in detoxification.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 ug of protein (determined by LAL method).
• Activity Specifications test method
  ≥ 20 U/mg as determined with BioVision’s GST colorimetric activity assay kit, Cat # K263-100.
• Biological activity
  ≥ 20 U/mg as determined with BioVision’s GST colorimetric activity assay kit, Cat # K263-100.
• Results
  ≥ 20 U/mg as determined with BioVision’s GST colorimetric activity assay kit, Cat # K263-100.
• Unit Definition
  One unit of the recombinant GST-Δ is defined as the amount of enzyme that conjugates 1.0 µmole of 1-chloro-2, 4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
• Molecular Weight
  26.4 kDa (236 aa, 1-216 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  2.0 mg/ml
• Appearance
  Liquid
• Physical form description
  2.0 mg/ml solution in 30% glycerol without additives.
• Background Information
  Glutathione S-transferase Δ is an enzyme in Bombyx mori (Silk moth) encoded by the GSTd2 gene. GSTs are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into nine classes: alpha, delta, kappa, mu, omega, pi, theta, zeta and microsomal. GSTs play a role in susceptibility to cancer, and other diseases.
• Amino acid sequence
  MGSSHHHHHHSSGLVPRGSH MTIDLYYVPG SAPCRAVLLT AKALNLNLNLKLVDLHHGEQLKPEYLKLNPQHTVPTLVDDG LSIWESRAIITYLVNKYAKGSSLYPEDPKARALVDQRLYFDIGTLYQRFSDYFYPQVFAGAPADKAKNEKVQ EALQLLDKFLEGQKYVAGPNLTVADLSLIASVSSLEASDIDFKKYAN VKRWYETVKSTAPGYQEANEKGLEAFKGLV NSMLKK
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GST-Δ/GSTD2   Active   Bombyx   mori   Silk   moth  
• Gene symbol
  MGST3
• Short name
  GST-Δ/GSTD2, Active Recombinant (Bombyx mori (Silk moth))
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  GST-Δ/GSTD2, functionnal Rec. (Bombyx mori (Silk moth))
• Alternative technique
  rec

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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