Nitrite Assay Kit (Griess Reagent)

• Catalog number
  K544-200
• Price
  Please ask
• Size
  200 assays

• Description
  Detects total nitrite
• Summary
  • Detection method- Absorbance (540 nm) • Species reactivity- Mammalian • Applications- BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.
• Detection Method
  Detection method- Absorbance (540 nm)
• Species Reactivity
  Eukaryotes
• Applications
  Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.
• Sample Type
  Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Features Benefits
  • Simple two-step procedure; takes only ~1-1.5 hours • Fast and convenient • BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of total nitrite.
• Storage Conditions
  4 °C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Nitrogen-based ions, nitrite and nitrate, are found in almost every living organism. Furthermore, endogenous Nitrite levels are found in mammals and also can be obtained from dietary sources. In humans, nitrite is further metabolized to Nitric Oxide and other reactive nitrogen species (nitrogen oxides). The Nitrate-Nitrite-NO biochemical pathway is well known for its participation in cell signaling, hypoxia-dependent response and regulation of blood flow. Recent studies suggest the reduction of Nitrite to Nitrogen Oxygen in the mitochondria. Specifically, myoglobin and xanthine oxidoreductase could generate NO under hypoxic conditions leading to mitochondrial respiration. BioVision’s Nitrite Assay Kit utilizes the Griess Reagent, a classic protocol for the estimation of nitrite. In the assay, nitrite is reduced to Nitrogen Oxide using Griess Reagent I. Then, Nitrogen Oxide reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at OD 540 nm. The one-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Nitrite   Kit   Griess   Reagent  
• Short name
  Nitrite Assay Kit (Griess Reagent)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Nitrite test reagent (Griess Reagent)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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