Granzyme B, Mouse recombinant (Insect)

  • Catalog number
    7608-5
  • Price
    Please ask
  • Size
    5 ug
  • Synonyms
    GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, mouse lymphocyte protein, HLP, C11
  • Alternative_names
    GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11
  • Description
    An active serine protease which processes pro-caspases to induce apoptosis
  • Recombinant
    Yes
  • Source
    Insect cell
  • Purity by SDS PAGE
    ≥90%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    ≥ 40 units/mg
  • Biological activity
    Activity of GZMB was tested using the synthetic peptide, IETD-AFC, as a substrate. GZMB releases AFC and then the GZMB activity can be quantified by fluorescent detection of free AFC at Ex/Em = 380/500 nm.
  • Unit Definition
    One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl2.
  • Molecular Weight
    33 kDa
  • Storage Temp
    -70°C
  • Shipping
    dry ice
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    rmGranzyme is supplied as a lyophilized powder
  • Reconstitution Instructions
    Use 50 µl sterile D.I. water to dissolve 5 µg protein to a final conc of 0.1 µg protein/µl.
  • Background Information
    Granzyme B (GZMB) is a serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and enters the target cell, assisted by perforin. With its unique substrate specificity (granzyme B cleaves after Asp), granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • About
    Insects (from Latin insect, a calque of Greek ἔντομον [éntomon], "cut into sections") are a class of invertebrates within the arthropod phylum that have a chitinous exoskeleton, a three-part body (head, thorax and abdomen), three pairs of jointed legs, compound eyes and one pair of antennae. They are the most diverse group of animals on the planet, including more than a million described species and representing more than half of all known living organisms. The number of extant species is estimated at between six and ten million, and potentially represent over 90% of the differing animal life forms on Earth. Insects may be found in nearly all environments, although only a small number of species reside in the oceans, a habitat dominated by another arthropod group, crustaceans. There are non competitive and competitive antagonists.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    GZMB
  • Short name
    Granzyme B, Mouse recombinant ( )
  • Technique
    Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    mouse
  • Species
    Insect, Insects, Mouses
  • Alternative name
    Granzyme B, Mouse Rec. (Insect)
  • Alternative technique
    rec, murine
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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