Granzyme B Antibody

• Catalog number
  V2564-100UG
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Granzyme B
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens). Other species not tested. ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  FACS, IF, WB, IHC-P
• Recommended dilutions
  Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 0.5-1ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
• Notes
  Optimal dilution of the Granzyme B antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Intented use
  This Granzyme B antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P10144
• Purity
  Purified by protein A Affinity Chromatography
• Description
  Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'non-self' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.
• Immunogen
  A recombinant fragment (amino acids 73-187) from the human protein was used as the immunogen for the Granzyme B antibody.
• Storage
  Store the Granzyme B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
• Localization
  Cytoplasmic
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Granzyme  
• Gene symbol
  GZMB
• Short name
  Anti-Granzyme B
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Granzyme B
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:4709
• Gene
  GZMB
• Long gene name
  granzyme B
• Synonyms gene
  • CTLA1
  • CSPB
• Synonyms gene name
  • granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Synonyms
  • CCPI
  • CGL-1
  • CSP-B
  • CGL1
  • CTSGL1
  • HLP
  • SECT
• Synonyms name
  • fragmentin 2
  • cytotoxic serine protease B
  • cathepsin G-like 1
  • T-cell serine protease 1-3E
  • granzyme 2
  • cytotoxic T-lymphocyte-associated serine esterase 1
• GenBank acession
  • BC030195
• Locus
  14q12
• Discovery year
  1989-05-12
• Entrez gene record
  3002
• Pubmed identfication
  • 2323780
• RefSeq identity
  • NM_004131
• Classification
  • Granule associated serine proteases of immune defence
• VEGA ID
  OTTHUMG00000029369

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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