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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
maltose binding protein (MBP) fusion of Zea mays GR, O64409
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG
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How to reconstitute
For reconstitution add 100 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL), immunoprecipitation (IP)
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Connected products
AS06 183 | anti-GS, glutathione synthase antibodiescollection of antibodies to stress proteins
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Recommended dilutions for use
1: 5000 with standard ECL (WB), needs to be optimized, 2 µg (IP), 1: 1000 (IL)
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Molecular weight expected аpparent
54 kDa
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Verified reactivity
Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Pisum sativum, Silene vulgaris, Solanum tuberosum, Zea mays, Scenedesmus quadricauda (algae)
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Possible reactivity
dicots including: Brassica rapa, Glycine max;monocots including: Oryza sativa, trees: Populus balsamifera
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody will recognize the chloroplastic and cytoplasmic forms of the enzyme.
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References
Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1-12. Shaw et al. (2015). β-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.). Environ Sci Pollut Res Int. 2015 Sep 29. Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4. Kovácik et al. (2013). Oxidative stress, uptake and bioconversion of 5-fluorouracil in algae. Chemosphere. 2013 Dec 28. pii: S0045-6535(13)01679-2. doi: 10.1016/j.chemosphere.2013.11.074. (immunolocalization in algae)Sobrino-Plata et al. (2013). Specific stress responses to cadmium, arsenic and mercury appear in the metallophyte Silene vulgaris when grown hydroponically. RSC Advances, Jan 24.
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Scientific context
Glutathione reductase (GR, EC 1.6.4.2) is an important enzyme for plant protection against environmental stress. It functions in plant defense reactions in the conversion of glutathione disulphide to reduced glutathione (GSH).
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Notes
total IgG concentration is 7 µg/ µl
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Protein number
O64409
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TAIR number
AT3G54660
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