Goat, Light® 488 conjugated, min. cross-reactivity to bovine, horse, mouse, rabbit serum proteins, mouse lgG1

  • Catalog number
    AS16 3352
  • Price
    Please ask
  • Size
    0.5 mg
  • Available ordering format
    Lyophilized
  • Immunogen
    Purified Human IgG, Fc fragment
  • Raised in
    Goat
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified
  • How to reconstitute
    For reconstitution add 0.55 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
  • Storage condition
    Store freeze-dried powder at 2-8 °C.
  • Verified applications
    Flow cytometry (FACS), Immunomicroscopy (IM)
  • Connected products
    Other secondary antibodies
  • Recommended dilutions for use
    1:20 - 1:2 000 (FACS and IM)
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    Goat anti-Human IgG Fc, F(ab)'2 Fragment.
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    To be added when available, antibody released in April 2016.
  • Scientific context
    Goat anti-Human IgG Fc, F(ab)'2 Fragment, DyLight® 488 conjugated, min. cross-reactivity to bovine, horse, mouse, rabbit serum proteins, mouse lgG1 is a secondary antibody conjugated to DyLight® 488, which binds to human IgG Fc, F(ab)'2 Fragment in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm.DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
  • Notes
    Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.Affinity purified using solid phase Human IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on human IgG . Based on IEP, no reactivity is observed to: - non-immunoglobulin human serum immunoglobulins - light chains on all human immunoglobulins - human IgG F(ab)'2 fragment - mouse IgG1 - serum proteins from bovine, horse, mouse, or rabbit .
  • Protein number
    O75015
  • TAIR number
    Refer to NCBI
  • Latin name
    Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus
  • Properties
    Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • About
    Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
  • Description
    Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
  • Group
    Sera
  • Gene target
    Light®   488   conjugated   min   cross   proteins   lgG1  
  • Short name
    Goat, Light® 488 conjugated, min. cross-reactivity , , mouse, rabbit serum proteins, mouse lgG1
  • Technique
    Rabbit, light, Mouse, serum, Goat, antibody Conjugates, goats, mouses
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
  • Species
    Bos Taurus genes are available form the Bovine Genome database. Goats, Horses, Mouses
  • Alternative name
    caprine, Light® 488 coupled, min. cross-This (antibody to-) recognizes to bovine, horse, mouse, production species: rabbit blood serum proteins, mouse lgG1
  • Alternative technique
    rabbit-anti, sera, murine, caprine
  • Tissue
    serum
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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