-
Available ordering format
Lyophilized
-
Immunogen
Purified human Kappa (k) Chain BAA37169.1
-
Raised in
Goat
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Affinity purified IgG
-
How to reconstitute
For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
-
Storage condition
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
-
Verified applications
Contact Gentaur support center
-
Connected products
other secondary antibodies against human kappa chain HRP conjugated
-
Recommended dilutions for use
This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5 000 for Immunohistochemistry 1:200-1:5000 for ELISA/Western blot
-
Molecular weight expected аpparent
see datasheet
-
Verified reactivity
to be determined
-
Possible reactivity
to be determined
-
No reactivity
to be determined
-
Supplementary information
-
References
Refer to PubMed
-
Scientific context
Goat anti-human kappa (k) chain is a secondary antibody conjugated to HRP, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
-
Notes
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
-
Protein number
Refer to NCBI
-
TAIR number
Refer to NCBI
-
-
Latin name
Capra aegagrus hircus
-
Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugation
HRP Conjugated