Goat, L), Biotin conjugated

  • Catalog number
    AS10 655
  • Price
    Please ask
  • Size
    2 mg
  • Available ordering format
    Liquid
  • Immunogen
    Purified Guinea pig IgG, whole molecule
  • Raised in
    Goat
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified IgG
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
  • Verified applications
    ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
  • Connected products
    no related to other products
  • Recommended dilutions for use
    1: 2000 - 20 000 (ELISA) and (WB), 1: 500 -1: 5000 (IHC)
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    guinea pig IgG heavy and light chains (H&L)
  • Possible reactivity
    guinea pig IgG heavy and light chains (H&L)
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    No reactivity is observed to non-immunoglobulin guinea pig serum proteins based in immunoelectrophoresis.This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.
  • References
    to be added when available
  • Scientific context
    Goat anti-guinea pig IgG is a secondary antibody conjugated to biotin which binds to all guinea pig immunoglobulins in immunological assays.
  • Notes
    biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Latin name
    Capra aegagrus hircus
  • Gene target
  • Short name
    Goat, L), conjugated
  • Technique
    Goat, antibody Conjugates, goats
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Label
    Biotin
  • Species
    Goats
  • Alternative name
    caprine, L), biotinilated coupled
  • Alternative technique
    caprine
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