Goat, Fc, Biotin conjugated, min. cross-reactivity bovine/mouse/rabbit serum

  • Catalog number
    AS10 794
  • Price
    Please ask
  • Size
    2 mg
  • Available ordering format
    Liquid
  • Immunogen
    Purified human IgG
  • Raised in
    Goat
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified goat IgG
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
  • Verified applications
    suitable for all immunoassays applications
  • Connected products
    antibodies to human immunoglobulins
  • Recommended dilutions for use
    optimal working dilution should be determined by the investigator
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    human IgG Fc (two heavy chains with constant domains)
  • Possible reactivity
    human IgG Fc (two heavy chains with constant domains)
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on human immunoglobulins, non-immunoglobulin human serum proteins or serum from bovine, mouse, rabbit based on immunoelectrophoresis.
  • References
    to be added when available
  • Scientific context
    Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to biotin which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
  • Notes
    biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Latin name
    Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • About
    Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
  • Description
    Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
  • Group
    Sera
  • Gene target
    conjugated   min   cross  
  • Short name
    Goat, Fc, conjugated, min. cross-reactivity /mouse/rabbit serum
  • Technique
    Rabbit, Mouse, serum, Goat, FC, antibody Conjugates, goats, mouses
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
  • Label
    Biotin
  • Species
    Mouse, Bos Taurus genes are available form the Bovine Genome database. Goats, Mouses
  • Alternative name
    caprine, fragment c, biotinilated coupled, min. cross-This (antibody to-) recognizes bovine/mouse/production species: rabbit blood serum
  • Alternative technique
    rabbit-anti, sera, murine, caprine
  • Tissue
    serum
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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