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Products_type
Secondary Antibody
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Products_short_name
[Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific)]
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Products_name_syn
[Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads - HRP; Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) (Minimal cross reactivity with human, rat, & mouse immunoglobulins)]
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Clonality
Polyclonal
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Reactivity
Rabbit
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Specificity
Reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human, rat, and mouse immunoglobulins.
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Purity
Gel filtration of pepsin-digested antibody
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Form
Horseradish Peroxidase (HRP) Conjugate
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Storage_stability
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
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Tested_application
ELISA; Immunoblot; Immunohistochemistry
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
f(ab')2s
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Latin name
Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short name
Goat F(ab')2 Anti-Rabbit IgG (H+L), Mouse/ / ads-
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Technique
F(ab')2, Rabbit, anti-, Mouse, anti, Goat, IgG, antibody to, antibodies, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. goats, IgGs, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
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Label
HRP
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Species
Human, Goats, Humans, Mouses, Rats
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Alternative name
caprine F(Antibody')2 Antibody tohost: rabbit Immunoglobulin G (heavy + light chain), Mouse/Rat/H. sapiens ads-horseradish peroxidase
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Alternative technique
secondairy, rabbit-anti, murine, antibodies, caprine, immunoglobulins
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