Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (Fc specific)

  • Catalog number
    GARa/Ig(Fc)/7S
  • Price
    Please ask
  • Size
    10 mg
  • Category
    Secondary Antibodies
  • Long description
    The defined antibody specificity is directed to the major isotypes of the rat immunoglobulin system, including antibody to common determinants and to class specific determinants as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5,000.
  • Antibody come from
    Highly purified IgG and pools of homogenous IgA and IgM isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Antigen antibody binding interaction
    Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (Fc specific) Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Rat
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has not been tested for cross-reactivity.
  • Antibody s specificity
    Purified IgG fraction of polyclonal Goat antiSerum to Rat immunoglobulins IgG, IgA, IgM, Fc specific.
  • Research interest
    Veterinary
  • Application
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
  • Antibody s suited for
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
  • Storage
    The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Latin name
    Capra aegagrus hircus, Rattus norvegicus
  • Properties
    Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Gene target
    IgG1   IgG2a   IgG2b   IgG2c   specific  
  • Short name
    Goat anti IgG1 IgG2a IgG2b IgG2c IgA IgM (Fc specific)
  • Technique
    anti, Goat, IgA, IgM, FC, antibody to, goats, IgAs, IgMs
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    not specified, IgA, IgG1, IgG2a, IgG2b, IgG2c, IgM
  • Label
    unlabeled
  • Species
    Rat, Goats, Rats
  • Alternative name
    caprine antibody to Rat IgG1 IgG2a IgG2b IgG2c Immunoglobulin A Immunoglobulin M (fragment c specific)
  • Alternative technique
    antibodies, caprine, immunoglobulin-a, immunoglobulin-m
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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