Goat anti-Rabbit IgG (H&L) -F(ab)'2 Fragment, DyLight650 Conjugate, min x w/bovine, human, or mouse lgG or serum proteins

  • Catalog number
    MBS688045
  • Price
    Please ask
  • Size
    5x0.5 mg
  • Products_type
    Secondary Antibody
  • Products_short_name
    [IgG (H&L), F(ab)'2 Fragment]
  • Products_name_syn
    [Goat anti-Rabbit IgG (H&L), F(ab)'2 Fragment - Affinity Pure]
  • Reactivity
    Rabbit. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, human, or mouse IgG from human or mouse
  • Specificity
    Based on IEP, this antibody reacts with: heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins
  • Purity
    > 90% based on SDS-PAGE, Small amounts of intact IgG may be present. Affinity purified using solid phase Rabbit IgG.
  • Form
    Lyophilized
  • Concentration
    1.0 mg/ml (E 1% at 280 nm = 13.0)
  • Storage_stability
    Store freeze-dried powder at 2-8 degree C. Shelf Life: Product is stable for up to 4 weeks at 2-8 degree C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 degree C.
  • Tested_application
    Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
  • Latin name
    Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Group
    Sera
  • Gene target
  • Short name
    Goat anti-Rabbit IgG (H L) -F(ab)'2 , DyLight650 Conjugate, min x w/ , , mouse lgG serum proteins
  • Technique
    Rabbit, anti-, Mouse, serum, anti, Goat, IgG, antibody to, antibodies, goats, IgGs, mouses
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
  • Isotype
    IgG, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. Heavy & Light chains, IgG
  • Species
    Mouse, Bos Taurus genes are available form the Bovine Genome database. Goats, Humans, Mouses
  • Alternative name
    caprine Antibody tohost: rabbit Immunoglobulin G (heavy + light chain) -F(Antibody)'2 Fragment, DyLight650 Conjugate, min x w/bovine, H. sapiens, and mouse lgG and blood serum proteins
  • Alternative technique
    rabbit-anti, sera, murine, antibodies, caprine, immunoglobulins
  • Tissue
    serum
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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