Goat anti Human secretory component free and bound

Goat anti Human secretory component free and bound is available 4 times from Nordc labs

GAHu/SC | Goat anti Human secretory component (free and bound)size: 1 mL | 246.14 USD

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GAHu/SC/Bio | Goat anti Human secretory component (free and bound) antibody conjugated with Biotinsize: 1 mL | 360.38 USD

  • Catalog number
  • Supplier
  • Price
    360.38 USD
  • Size
    1 mL
  • Long description
    Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The immunoconjugate does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In immunocytochemical and immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in human body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:8,000.
  • Antibody come from
    Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM. Purified free human secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Antigen-antibody binding interaction
    Goat anti Human secretory component (free and bound) antibody conjugated with Biotin Antibody
  • Antibody's reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
  • Antibody's specificity
    Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Human secretory component, free and bound
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
  • Antibody's suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
  • Storage
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
  • Properties
    If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugation
  • Latin name
    Capra aegagrus hircus
  • French translation
  • Gene target
  • Short name
    Goat anti secretory component (free bound) antibody conjugated with
  • Technique
    anti human, Antibody, anti, Goat
  • Label
  • Alternative name
    caprine antibody to H. sapiens secretory component (free and bound) (Antibody to) coupled including biotinilated
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
Additional information

GAHu/SC/FITC | Goat anti Human secretory component (free and bound) antibody conjugated with FITCsize: 1 mL | 360.38 USD

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GAHu/SC/PO | Goat anti Human secretory component (free and bound) antibody conjugated with Horseradish peroxidasesize: 1 mL | 471.08 USD

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  • Category
    Secondary Antibodies
  • Clone
  • Antibody is raised in
  • Antibody's reacts with
  • Research interest
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Host
  • Species
  • Alternative technique
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Goat anti Human secretory component free and bound
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