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Immunogen
Peptide derived from human Nrf2 protein (a.a. 445-458)
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Antibody s target
Human Nrf2
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Antibody s full description
Goat Anti-Human Nrf2 Polyclonal
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Primary research fields
Cancer, Oxidative Stress, Cell Signaling, Epigenetics, Cardiovascular System, Heart
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Erythroid derived 2 antibody, HEBP1 antibody, like 2 antibody, NF E2 related factor 2 antibody, NF-E2-related factor 2 antibody, NF2L2_HUMAN antibody, NFE2 related factor 2 antibody, NFE2-related factor 2 antibody, NFE2L2 antibody, Nrf 2 antibody, NRF2 antibody, Nuclear factor (erythroid derived 2) like 2 antibody, Nuclear factor antibody, nuclear factor erythroid 2 like 2 antibody, Nuclear factor erythroid 2 related factor 2 antibody, Nuclear factor erythroid 2-related factor 2 antibody, Nuclear factor erythroid derived 2 like 2 antibody
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Verified applications
WB, ELISA
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Raised in
Goat
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Antibody s reactivity
Human, Mouse, Rat, Bovine, Dog, Pig
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Antibody s recommended dilutions for use
WB (1:1500), ELISA (1:64000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
TBS pH7.3, 0.02% sodium azide, 0.5% BSA
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Aggrephagy associated oxidative stress response transcription factor.
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1500 dilution of SPC-218 was sufficient for detection of Nrf2 in 35 µg of human lung lysate by electrochemiluminescence analysis using donkey anti-goat IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Nrf2 is a transcription factor which plays a role in the oxidative stress response. Under normal cellular conditions, it is sequestered in the cytoplasm by Keap1 and Cullin3. When under oxidative stress, Nrf2 is release from Keap1 by p62 and is translocated to the nucleus where it acts as a transcription factor for the expression of oxidative stress-related products.
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Bibliography
1. Itoh K., Wakabayashi N., Katoh Y., Ishii T., Igarashi K., Engel J., et al. (1999) Genes Dev. 13(1): 76-86.
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Released date
12/Jun/2014
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NCBI number
NP_006155.13
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Gene number
4791
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Protein number
Q16247
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.05
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
NFE2L2, GABPA
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Short name
Goat Anti- Nrf2 Polyclonal:
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Technique
anti-Human, Polyclonal, anti-, anti, Goat, antibody to, antibodies, antibodies against human proteins, goats, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
Immunoglobulin
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Label
Biotin
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Species
Human, Goats, Humans
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Alternative name
caprine Antibody toHuman Nrf2 polyclonal: biotinilated
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Alternative technique
polyclonals, antibodies, caprine
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