Goat Anti-Equine IgG (H+L)-FITC

• Catalog number
  MBS674803
• Price
  Please ask
• Size
  5x1 mg

• Products_type
  Secondary Antibody
• Products_short_name
  [Goat Anti-Equine IgG (H+L)]
• Products_name_syn
  [Goat Anti-Equine IgG(H+L) - FITC; Goat Anti-Equine IgG (H+L)]
• Clonality
  Polyclonal
• Reactivity
  Equine
• Specificity
  Reacts with the heavy and light chains of equine IgG as demonstrated by ELISA.May also react with the light chains of other equine immunoglobulins.
• Purity
  Affinity chromatography on equine IgG covalently linked to agarose.
• Form
  Fluorescein (FITC) Conjugate
• Storage_stability
  The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at - 20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
• Tested_application
  ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  Equine   H+L  
• Short name
  Goat Anti-Equine IgG (H+L)-FITC
• Technique
  anti-, anti, FITC, Goat, IgG, antibody to, antibodies, Fluorescein, goats, IgGs
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  IgG, IgG
• Label
  FITC
• Species
  Goats
• Alternative name
  caprine Antibody tohorse Immunoglobulin G (heavy + light chain)-fluorecein
• Alternative technique
  antibodies, caprine, fluorescine, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products