Glucose and Sucrose Colorimetric/Fluorometric Assay Kit
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Catalog number
K616-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method-Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian
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Sample Type
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring glucose level during fermentation and glucose feeding in protein expression processes
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Features Benefits
• Simple procedure; takes ~40 minutes • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of glucose and sucrose individually in various biological samples
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Glucose (C₆H₁₂O₆; FW: 180.16) and sucrose (C₁₂H₂₂O₁₁; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase. BioVision’s Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (O.D. 570 nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Glucose Sucrose Colorimetric/Fluorometric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Glucose and Sucrose colorimetry/fluorescence test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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