Glucose and Sucrose Colorimetric/Fluorometric Assay Kit

  • Catalog number
    K616-100
  • Price
    Please ask
  • Size
    100 assays
  • Summary
    • Detection method-Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian
  • Sample Type
    Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring glucose level during fermentation and glucose feeding in protein expression processes
  • Features Benefits
    • Simple procedure; takes ~40 minutes • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of glucose and sucrose individually in various biological samples
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Glucose (C₆H₁₂O₆; FW: 180.16) and sucrose (C₁₂H₂₂O₁₁; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase. BioVision’s Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (O.D. 570 nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.
  • Test
    Biovision supplies other types of Assays as 1.
  • Properties
    Colorimetric assays or detection use UV absorption or enzymatic color reaction.
  • Gene target
  • Short name
    Glucose Sucrose Colorimetric/Fluorometric Assay Kit
  • Technique
    colorimetric, Assay, assays, colorimetry
  • Host
    Assay
  • Alternative name
    Glucose and Sucrose colorimetry/fluorescence test reagent
  • Alternative technique
    assays, arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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