Calmodulin, human recombinant

• Catalog number
  7838-500
• Price
  Please ask
• Size
  500 ug

• Synonyms
  CaM, CALM Phosphodiesterase 3':5'-cyclic nucleotide activator, CALM2, PHKD, CAMII, PHKD2, phosphorylase kinase delta.
• Alternative_names
  CaM, CALM Phosphodiesterase 3':5'-cyclic nucleotide activator, CALM2, PHKD, CAMII, PHKD2, phosphorylase kinase delta.
• Description
  ≥95% Pure active Human recombinant Calmodulin which can bind to and regulate a multitude of protein targets.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  The Ca2+ binding affinity of rh Calmodulin was evidenced by the electrophoretic mobility shift of Calmodulin in the presence of calcium and EDTA. Calmodulin has been shown to migrate differently in the presence and absence of Calcium. Ref.: Proc Natl Acad Sci U S A. 2004 Apr 6;101 (14):4787-92.
• Biological activity
  The Ca2+ binding affinity of rh Calmodulin was evidenced by the electrophoretic mobility shift of Calmodulin in the presence of calcium and EDTA. Calmodulin has been shown to migrate differently in the presence and absence of Calcium. Ref.: Proc Natl Acad Sci U S A. 2004 Apr 6;101 (14):4787-92.
• Molecular Weight
  16.8 kDa
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from a salt free solution.
• Reconstitution Instructions
  Reconstitute in water or an appropriate buffer (TBS, PBS, etc)
• Background Information
  Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. Calmodulin is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. Calmodulin can also make use of the calcium stores in the endoplasmic reticulum, and the sarcoplasmic reticulum. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.
• Amino acid sequence
  MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Calmodulin  
• Gene symbol
  CALML5, CALM3, CALML6
• Short name
  Calmodulin, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Calmodulin, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:18180
• Gene
  CALML5
• Long gene name
  calmodulin like 5
• Synonyms gene name
  • calmodulin-like 5
• Synonyms
  • CLSP
• Synonyms name
  • calmodulin-like skin protein
• GenBank acession
  • AF172852
• Locus
  10p15.1
• Discovery year
  2003-11-10
• Entrez gene record
  51806
• Pubmed identfication
  • 10777582
• RefSeq identity
  • NM_017422
• Classification
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000017598

Gene info

• Identity
  HGNC:1449
• Gene
  CALM3
• Long gene name
  calmodulin 3
• Synonyms gene name
  • calmodulin 3 (phosphorylase kinase, delta)
• Synonyms
  • PHKD
• Synonyms name
  • prepro-calmodulin 3
  • phosphorylase kinase subunit delta
• Locus
  19q13.32
• Discovery year
  1991-06-05
• Entrez gene record
  808
• Classification
  • Receptor ligands
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000133517
• Locus Specific Databases
  • LRG_1082

Gene info

• Identity
  HGNC:24193
• Gene
  CALML6
• Long gene name
  calmodulin like 6
• Synonyms gene name
  • calmodulin-like 6
• Synonyms
  • CAGLP
• GenBank acession
  • AF490905
• Locus
  1p36.33
• Discovery year
  2005-01-24
• Entrez gene record
  163688
• RefSeq identity
  • NM_138705
• Classification
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000000943

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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