GFP Tag (168AT1211) Monoclonal Antibody

• Catalog number
  bsm-51005M
• Price
  Please ask
• Size
  0.1ml

• Long name
  GFP Tag (168AT1211) Monoclonal Antibody
• Also known as
  GFP Tag (168AT1211) Monoclonal Antibody
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  GFP Tag
• Specificity
  This is a highly specific antibody against GFP Tag.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Monoclonal
• Clone number
  168AT1211
• Source
  Purified His-tagged GFP protein was used to produced this monoclonal antibody.
• Tested Applications
  WB
• Recommended dilutions
  WB(1:2000)
• Cross reactivity
  Human, Other Species
• Cross reactive species details
  All Species
• Background of the target antigen
  Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.
• Purification method
  Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  GFP   Tag   168AT1211   Monoclonal  
• Short name
  Anti-GFP Tag
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
• Host
  Mouse (Mus musculus)
• Isotype
  Immunoglobulin G (IgG)1
• Label
  gfp
• Alternative name
  GFP Tag (168AT1211) Monoclonal Antibody
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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