GDP, Galactose Phosphorylase

  • Catalog number
    AS10 723
  • Price
    Please ask
  • Size
    200 µl
  • Available ordering format
    Lyophilized
  • Immunogen
    KLH-conjuated synthetic peptide derived from known GDP-L-Galactose Phosphorylase sequences, including Arabidopsis thaliana Q8LKQ7 (At4g26850) and Chlamydomonas reinhardtii
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Serum
  • How to reconstitute
    For reconstitution add 200 µl of sterile water.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    Plant and algal protein extraction buffer Secondary antibodies
  • Recommended dilutions for use
    1 : 1200 with alkaline phosphatase, 1: 3000 with regular ECL (WB)
  • Molecular weight expected аpparent
    51 | 50 kDa
  • Verified reactivity
    Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii
  • Possible reactivity
    dictos: Sorghum bicolor, monocots: Oryza sativa, moss: Physcomitrella patens
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    to be added when available
  • References
    to be added when available, antibody available in July 2010
  • Scientific context
    GDP-L-Galactose Phosphorylase is a histidine triad (HIT) enzyme of the Smirnoff-Wheeler pathway of ascorbic acid synthesis in plants. Encoded by VTC2 gene. The enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in a reaction that consumes inorganic phosphate and produces GDP.
  • Notes
    None
  • Protein number
    Q8LKQ7
  • TAIR number
    AT4G26850
  • Gene target
  • Short name
    GDP, Galactose Phosphorylase
  • Alternative name
    GDP, Galactose Phosphorylase
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