GCN2 Antibody

• Catalog number
  PA2028
• Price
  Please ask
• Size
  0,1 mg

• Target antigen
  GCN2
• Clonality
  Polyclonal antibody
• Clone
  Polyclonal antibody
• Raised in
  rabbit
• Type of the antibody
  IgG polyclonal antibody
• Product form
  freeze-dried
• Reacts with species
  human
• Analyses
  WB
• Immunogen
  A synthetic peptide corresponding to a sequence in the middle region of mouse GCN2(868-886aa ATDHLAFTAEGKQDDQAGD), different from the related rat sequence by one amino acid.
• Product configuration
  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Purification
  Immunogen affinity purified.
• Solubilization
  The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
• Storage condtions
  Keep the GCN2 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
• Tips
  The GCN2 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
• Background
  EIF2AK4(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4), also called GCN2, is an enzyme that in humans is encoded by the EIF2AK4 gene. EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. Hartz(2005) mapped the EIF2AK4 gene to chromosome 15q15.1 based on an alignment of the EIF2AK4 sequence with the genomic sequence. Berlanga et al.(1999) demonstrated that Gcn2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro. Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse Eif2ak4. Costa-Mattioli et al.(2005) reported a unique feature of hippocampal slices from Gcn2-null mice: in CA1, a single 100-Hz train induced a strong and sustained long-term potentiation(late LTP or L-LTP), which was dependent on transcription and translation.
• Related articles
  1. Berlanga, J. J., Santoyo, J., de Haro, C. Characterization of a mammalian homolog of the GCN2 eukaryotic initiation factor 2-alpha kinase. Europ. J. Biochem. 265: 754-762, 1999. 2. Costa-Mattioli, M., Gobert, D., Harding, H., Herdy, B., Azzi, M., Bruno, M., Bidinosti, M., Ben Mamou, C., Marcinkiewicz, E., Yoshida, M., Imataka, H., Cuello, A. C., Seidah, N., Sossin, W., Lacaille, J.-C., Ron, D., Nader, K., Sonenberg, N. Translational control of hippocampal synaptic plasticity and memory by the elF2-alpha kinase GCN2. Nature 436: 1166-1170, 2005. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 3/28/2005.
• Gene Name
  EIF2AK4
• Protein Name
  eIF-2-alpha kinase GCN2
• Gene Full Name
  eukaryotic translation initiation factor 2 alpha kinase 4
• Synonyms
  E2AK4_HUMAN antibody|Eif2ak4 antibody|Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody|Eukaryotic translation initiation factor 2-alpha kinase 4 antibody|GCN2 antibody|GCN2 eIF2alpha kinase antibody|GCN2 like protein antibody|GCN2-like protein antibody|KIAA1338 antibody|MGCN2 antibody
• Uniprot ID
  Q9QZ05
• Properties
  If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  GCN2  
• Gene symbol
  EIF2AK4
• Short name
  GCN2 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Alternative name
  GCN2 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:19687
• Gene
  EIF2AK4
• Long gene name
  eukaryotic translation initiation factor 2 alpha kinase 4
• Synonyms
  • GCN2
  • KIAA1338
• GenBank acession
  • AB037759
• Locus
  15q15.1
• Discovery year
  2002-11-11
• Entrez gene record
  440275
• Pubmed identfication
  • 10504407
• VEGA ID
  OTTHUMG00000172403

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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