GCLC Antibody
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Catalog numberbs-8402R
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PricePlease ask
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Size0.1ml
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Long nameGCLC Primary Polyclonal Antibody
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Also known asGCLC PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenGCLC
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SpecificityThis is a highly specific antibody against GCLC.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human GCLC
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Gene ID number2730
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenThe GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels.Defects in GCLC are the cause of hemolytic anemia.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesGamma ECS; Gamma GCS; Gamma glutamylcysteine synthetase; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCL; GCLC; GCLM; GCS; GCS Heavy Chain antibody GLCL; GLCLC; GLCLR; Glutamate cysteine ligase catalytic subunit; Glutamate cysteine ligase gamma glutamylcysteine synthetase regulatory 30.8kD; Glutamate cysteine ligase catalytic subunit; Glutamate cysteine ligase modier subunit; Glutamate cysteine ligase regulatory protein; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase catalytic subunit; GSH1_HUMAN.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolGCLC
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Short nameAnti-GCLC
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameGCLC Polyclonal Antibody
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Alternative techniqueantibodies
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Alternative to gene targetglutamate-cysteine ligase, catalytic subunit, GCL and GCS and GLCL and GLCLC, GCLC and IDBG-91041 and ENSG00000001084 and 102724876,2729, coenzyme binding, Cytoplasm, Gclc and IDBG-186028 and ENSMUSG00000032350 and 14629, GCLC and IDBG-628748 and ENSBTAG00000015571 and 512468
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Gene info
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Identity
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Gene
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Long gene nameglutamate-cysteine ligase catalytic subunit
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Synonyms gene
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Synonyms gene name
- glutamate-cysteine ligase, catalytic subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-24
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data