GAPDH, mouse recombinant
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Catalog numberP1099-10
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PricePlease ask
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Size10 ug
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SynonymsG3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2
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Alternative_namesG3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2
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DescriptionInvolved in glycolysis
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Activity Specifications test methodMeasured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
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Biological activitySpecific activity is > 40 units/mg, and is defined as theamount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
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Results> 40 units/mg
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Unit DefinitionOne unit is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
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Molecular Weight38.2kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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Concentration0.25 mg/ml
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AppearanceLiquid
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Physical form descriptionIn Phosphate buffer saline (pH 7.4)containing 20% glycerol 1mM DTT.
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Background InformationGapdh, also known as glyceraldehyde 3-phosphate dehydrogenase, is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, Gapdh has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. Recombinant mouse Gapdh, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MGSMVKVGVN GFGRIGRLVT RAAICSGKVE IVAINDPFID LNYMVYMFQY DSTHGKFNGT VKAENGKLVI NGKPITIFQE RDPTNIKWGE AGAEYVVEST GVFTTMEKAG AHLKGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGAAQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTPN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTED QVVSCDFNSN SHSSTFDAGA GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolGAPDH-DT, GAPDH, GAPDHS, GAPDHP15
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Short nameGAPDH, mouse recombinant
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Hostmouse
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SpeciesMouse, Mouses
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Alternative nameGAPDH, mouse Rec.
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Alternative techniquerec, murine
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Gene info
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Identity
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Gene
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Long gene nameGAPDH divergent transcript
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Locus
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Discovery year2021-03-12
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Entrez gene record
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Classification
- Divergent transcripts
Gene info
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Identity
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Gene
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Long gene nameglyceraldehyde-3-phosphate dehydrogenase
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Synonyms gene
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglyceraldehyde-3-phosphate dehydrogenase, spermatogenic
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglyceraldehyde-3-phosphate dehydrogenase pseudogene 15
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Synonyms gene
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Synonyms
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Locus
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Discovery year2003-11-26
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Entrez gene record
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data