GAPDH, mouse recombinant

  • Catalog number
    P1099-10
  • Price
    Please ask
  • Size
    10 ug
  • Synonyms
    G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2
  • Alternative_names
    G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2
  • Description
    Involved in glycolysis
  • Recombinant
    Yes
  • Source
    E. Coli
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    Measured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
  • Biological activity
    Specific activity is > 40 units/mg, and is defined as theamount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
  • Results
    > 40 units/mg
  • Unit Definition
    One unit is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
  • Molecular Weight
    38.2kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel pack
  • Shelf Life
    12 months
  • Concentration
    0.25 mg/ml
  • Appearance
    Liquid
  • Physical form description
    In Phosphate buffer saline (pH 7.4)containing 20% glycerol 1mM DTT.
  • Background Information
    Gapdh, also known as glyceraldehyde 3-phosphate dehydrogenase, is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, Gapdh has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. Recombinant mouse Gapdh, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
  • Amino acid sequence
    MGSSHHHHHH SSGLVPRGSH MGSMVKVGVN GFGRIGRLVT RAAICSGKVE IVAINDPFID LNYMVYMFQY DSTHGKFNGT VKAENGKLVI NGKPITIFQE RDPTNIKWGE AGAEYVVEST GVFTTMEKAG AHLKGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGAAQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTPN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTED QVVSCDFNSN SHSSTFDAGA GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    GAPDH  
  • Gene symbol
    GAPDH-DT, GAPDH, GAPDHS, GAPDHP15
  • Short name
    GAPDH, mouse recombinant
  • Technique
    Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    mouse
  • Species
    Mouse, Mouses
  • Alternative name
    GAPDH, mouse Rec.
  • Alternative technique
    rec, murine
Gene info
  • Identity
  • Gene
  • Long gene name
    GAPDH divergent transcript
  • Locus
  • Discovery year
    2021-03-12
  • Entrez gene record
  • Classification
    • Divergent transcripts
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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