GAPDH Loading Control Antibody
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Catalog number
R31532
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
GAPDH Loading Control
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 0.5-1ug/ml
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Notes
Titration of the GAPDH loading control antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This GAPDH loading control antibodyis to be used only for research purposes and not for diagnostics..
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Gene ID
2597
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Purity
Antigen affinity
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Description
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD).GAPDH antibody is commonly used as a western blot loading control.
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Immunogen
Amino acids YSNRVVDLMAHMASKE were used as the immunogen for this GAPDH loading control antibody.
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Storage
After reconstitution, the GAPDH loading control antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Localization
Nuclear and cytoplasmic
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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French translation
anticorps
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Gene target
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Gene symbol
GAPDH-DT, GAPDH, GAPDHS, GAPDHP15
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Short name
Anti-GAPDH Loading Control
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Technique
Antibody, Control, antibodies against human proteins, antibodies for, controls
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Isotype
Rabbit IgG
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Alternative name
Antibodies to GAPDH Loading Control
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Alternative technique
antibodies, controls
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Tissue
control
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Gene info
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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