GAPDH Antibody

• Catalog number
  A00227-1
• Price
  Please ask
• Size
  0,1 mg

• Target antigen
  GAPDH
• Clonality
  Polyclonal antibody
• Clone
  Polyclonal antibody
• Raised in
  rabbit
• Type of the antibody
  IgG polyclonal antibody
• Product form
  freeze-dried
• Reacts with species
  mouse
• Analyses
  WB,IHC-P
• Immunogen
  E.coli-derived human GAPDH recombinant protein (Position: N136-E335). Human GAPDH shares 95% and 94.5% amino acid (aa) sequence identity with mouse and rat GAPDH, respectively.
• Product configuration
  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Purification
  Immunogen affinity purified.
• Solubilization
  The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
• Storage condtions
  Keep the GAPDH Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
• Tips
  The GAPDH Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
• Background
  Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus.
• Related articles
  1. Bae, B.-I., Hara, M. R., Cascio, M. B., Wellington, C. L., Hayden, M. R., Ross, C. A., Ha, H. C., Li, X.-J., Snyder, S. H., Sawa, A. Mutant Huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH. Proc. Nat. Acad. Sci. 103: 3405-3409, 2006. 2. Colell, A., Ricci, J.-E., Tait, S., Milasta, S., Maurer, U., Bouchier-Hayes, L., Fitzgerald, P., Guio-Carrion, A., Waterhouse, N. J., Li, C. W., Mari, B., Barbry, P., Newmeyer, D. D., Beere, H. M., Green, D. R. GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation. Cell 129: 983-997, 2007. Note: Erratum: Cell 130: 385 only, 2007. 3. Tarze A, Deniaud A, Le Bras M, Maillier E, Molle D, Larochette N, Zamzami N, Jan G, Kroemer G, Brenner C (April 2007). "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization".Oncogene 26 (18): 2606–20.
• Gene Name
  GAPDH
• Protein Name
  Glyceraldehyde-3-phosphate dehydrogenase
• Gene Full Name
  glyceraldehyde-3-phosphate dehydrogenase
• Synonyms
  EC 1.2.1.12 | EC1.2.1.12 | G3P | GAPD | GAPDH | P04406
• Uniprot ID
  P04406
• Entrez GeneID
  2597
• Properties
  If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  GAPDH  
• Gene symbol
  GAPDH-DT, GAPDH, GAPDHS, GAPDHP15
• Short name
  GAPDH Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Alternative name
  GAPDH (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:55492
• Gene
  GAPDH-DT
• Long gene name
  GAPDH divergent transcript
• Locus
  12p13.31
• Discovery year
  2021-03-12
• Entrez gene record
  112268089
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:4141
• Gene
  GAPDH
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase
• Synonyms gene
  • GAPD
• GenBank acession
  • AF261085
• Locus
  12p13.31
• Discovery year
  2001-06-22
• Entrez gene record
  2597
• Pubmed identfication
  • 3170585
• RefSeq identity
  • NM_002046
• VEGA ID
  OTTHUMG00000137379

Gene info

• Identity
  HGNC:24864
• Gene
  GAPDHS
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Synonyms gene
  • GAPDS
• Synonyms
  • GAPDH-2
  • GAPD2
• GenBank acession
  • AJ005371
• Locus
  19q13.12
• Discovery year
  2004-02-10
• Entrez gene record
  26330
• Pubmed identfication
  • 10714828
• RefSeq identity
  • NM_014364
• VEGA ID
  OTTHUMG00000182298

Gene info

• Identity
  HGNC:13950
• Gene
  GAPDHP15
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase pseudogene 15
• Synonyms gene
  • GAPDP15
• Synonyms
  • GAPDH-Lp
  • b55C20.4
• Locus
  6p11.2
• Discovery year
  2003-11-26
• Entrez gene record
  642317
• VEGA ID
  OTTHUMG00000014927

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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