GAPDH, Active, human recombinant

• Catalog number
  P1100-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  Glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD
• Alternative_names
  Glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD
• Description
  Involved in glycolysis
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C
• Biological activity
  Specific activity is > 90 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
• Results
  > 90 units/mg
• Unit Definition
  One unit is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
• Molecular Weight
  36 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20% glycerol
• Background Information
  Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GAPDH   Active  
• Gene symbol
  GAPDH-DT, GAPDH, GAPDHS, GAPDHP15
• Short name
  GAPDH, Active, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  GAPDH, functionnal, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:55492
• Gene
  GAPDH-DT
• Long gene name
  GAPDH divergent transcript
• Locus
  12p13.31
• Discovery year
  2021-03-12
• Entrez gene record
  112268089
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:4141
• Gene
  GAPDH
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase
• Synonyms gene
  • GAPD
• GenBank acession
  • AF261085
• Locus
  12p13.31
• Discovery year
  2001-06-22
• Entrez gene record
  2597
• Pubmed identfication
  • 3170585
• RefSeq identity
  • NM_002046
• VEGA ID
  OTTHUMG00000137379

Gene info

• Identity
  HGNC:24864
• Gene
  GAPDHS
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Synonyms gene
  • GAPDS
• Synonyms
  • GAPDH-2
  • GAPD2
• GenBank acession
  • AJ005371
• Locus
  19q13.12
• Discovery year
  2004-02-10
• Entrez gene record
  26330
• Pubmed identfication
  • 10714828
• RefSeq identity
  • NM_014364
• VEGA ID
  OTTHUMG00000182298

Gene info

• Identity
  HGNC:13950
• Gene
  GAPDHP15
• Long gene name
  glyceraldehyde-3-phosphate dehydrogenase pseudogene 15
• Synonyms gene
  • GAPDP15
• Synonyms
  • GAPDH-Lp
  • b55C20.4
• Locus
  6p11.2
• Discovery year
  2003-11-26
• Entrez gene record
  642317
• VEGA ID
  OTTHUMG00000014927

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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