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Target antigen
gammaTubulin
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Clonality
Monoclonal antibody
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Clone
GTU-88
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Raised in
mice
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Type of the antibody
IgG1 monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Human
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Analyses
WB,ICC
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Immunogen
Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH.
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the gammaTubulin Antibody ( GTU)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The gammaTubulin Antibody ( GTU) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.
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Related articles
1. Rommens, J. M.; Durocher, F.; McArthur, J.; Tonin, P.; LeBlanc, J.-F.; Allen, T.; Samson, C.; Ferri, L.; Narod, S.; Morgan, K.; Simard, J. : Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21. Genomics 28: 530-542, 1995. 2. Zheng, Y.; Jung, M. K.; Oakley, B. R. : Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65: 817-823, 1991. 3. Stearns, T.; Evans, L.; Kirschner, M. : Gamma-tubulin is a highly conserved component of the centrosome. Cell 65: 825-836, 1991.
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Gene Name
GGA1; GGA2; GGA3
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Protein Name
ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3
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Gene Full Name
golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3
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Synonyms
VEAR|KIAA1080|KIAA0154|Gamma-adaptin-related protein 1|Gamma-adaptin-related protein 2|Golgi-localized, gamma ear-containing, ARF-binding protein 2|VHS domain and ear domain of gamma-adaptin
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Uniprot ID
Human(Q9UJY5), Mouse(Q8R0H9), Rat(Q5FVF3), Human(Q9UJY4), Mouse(Q6P5E6), Rat(Q4G038), Human(Q9NZ52), Mouse(Q8BMI3), Rat(D3ZHG8)
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Entrez GeneID
Human(26088), Mouse(106039), Rat(300066), Human(23062), Mouse(74105), Rat(293455), Human(23163), Mouse(260302), Rat(360658)
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
