Galactose and Lactose Colorimetric/Fluorometric Assay Kit

• Catalog number
  K617-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian
• Sample Type
  Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring levels during fermentation and feeding in protein expression processes
• Features Benefits
  • Simple procedure; takes ~ 1 hour • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of galactose and lactose individually in various biological samples
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Galactose (C₆H₁₂O₆ FW: 180.16) and lactose (C₁₂H₂₂O₁₁ FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. BioVision’s Galactose / Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (O.D 570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose – Free Galactose.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Galactose   Lactose   Fluorometric   Kit  
• Short name
  Galactose Lactose Colorimetric/Fluorometric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Galactose and Lactose colorimetry/fluorescence test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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