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Target antigen
GADD45A
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
Human, Mouse, Rat. No cross reactivity with other proteins.
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GADD45A(129-143aa NPHSSQWKDPALSQL), identical to the related mouse and rat sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the GADD45A Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The GADD45A Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Growth arrest and DNA-damage-inducible protein GADD45 alpha(GADD45A) is a protein that in humans is encoded by the GADD45A gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. Papathanasiou et al.(1991) demonstrated that the GADD45 gene is located on 1p34-p12. Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165-amino acid polypeptide. Northern blot analysis detected moderate expression of a 1.4-kb GADD45A transcript in heart, skeletal muscle, and kidney, with little or no expression in brain, placenta, lung, liver, and pancreas. Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.
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Related articles
1. Papathanasiou MA, Kerr NC, Robbins JH, McBride OW, Alamo I Jr, Barrett SF, Hickson ID, Fornace AJ Jr (Mar 1991). "Induction by ionizing radiation of the gadd45 gene in cultured human cells: lack of mediation by protein kinase C". Mol Cell Biol 11 (2): 1009–16. 2. Barreto, G., Schafer, A. Marhold, J., Stach, D., Swaminathan, S. K., Handa, V., Doderlein, G., Maltry, N., Wu, W., Lyko, F., Niehrs, C.Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.Nature445: 671-675, 2007.
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Gene Name
GADD45A
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Protein Name
Growth arrest and DNA damage-inducible protein GADD45 alpha
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Gene Full Name
growth arrest and DNA-damage-inducible, alpha
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Synonyms
DDIT 1 antibody|DDIT-1 antibody|DDIT1 antibody|DNA damage inducible transcript 1 antibody|DNA damage-inducible transcript 1 protein antibody|GA45A_HUMAN antibody|GADD45 antibody|GADD45A antibody|Growth arrest and DNA damage inducible 45 alpha antibody|Growth arrest and DNA damage inducible alpha antibody|Growth arrest and DNA damage-inducible protein GADD45 alpha antibody
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Uniprot ID
P24522
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
