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Available ordering format
Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
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Immunogen
Purified, full length native protein P02751
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB)
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Connected products
IMS06-060-315 | anti-Fibronectin, biotinylated antibodySecondary antibodies
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Recommended dilutions for use
1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent
263 kDa
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Verified reactivity
human
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Possible reactivity
bovine, mouse
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
only one precipitin arc in immunodiffusion against normal human serum or human fibronectin. Only one precipitin arc in immunoelectrophoresis against normal human serum or human fibronectin. Specific in immunoblotting against human fibronectin
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References
to be added when available
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Scientific context
Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. It occurs as dimer of two 200 kDa subunits. Fibronectin has binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.
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Notes
affinity purified IgY on human fibronectin agarose
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Protein number
P02751
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TAIR number
Refer to NCBI
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