Fluorescein Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Donkey]
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Catalog number
MBS417912
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Price
Please ask
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Size
0.25 mg
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Products_type
Secondary Antibody
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Products_short_name
[IgG [H&L]]
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Products_gene_name
[IgG]
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Reactivity
Rabbit
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Concentration
1.0 mg/ml (by UV absorbance at 280 nm)
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Storage_stability
This product is stable for several weeks at 4 degree C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
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Tested_application
Immunomicroscopy, Flow Cytometry (FC/FACS), Other antibody based fluorescent assays requiring lot-to-lot consistency.
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Test
A high affinity purification column was use to purify Fluorescein Conjugated anti-Rabbit IgG [H L] [ ] by MyBioSource by chromatographic size exclusion.
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Description
1 of protein A or G purified by MyBioSource will give more specificity than crude serum. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Equus africanus asinus, Oryctolagus cuniculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene target
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Short name
Fluorescein Conjugated anti-Rabbit IgG [H L] [ ]
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Technique
affinity, purified, Rabbit, anti-, anti, IgG, antibody to, antibodies, antibody Conjugates, IgGs, purifications
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
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Isotype
IgG, Heavy & Light chains, IgG
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Species
Donkeys
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Alternative name
Fluorescein coupled protein enriched Antibody tohost: rabbit Immunoglobulin G [heavy + light chain] [Donkey]
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Alternative technique
pure, rabbit-anti, antibodies, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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