OVA Conjugated FLAG Octapeptide (FLAG)
-
Catalog number
RPU50033-100ug
-
Price
Please ask
-
Size
100ug
-
-
Estimated Turnaround Time
6-11 business days
-
Gene Name
NA
-
Alternative Names
FLAG epitope; FLAG-Tag
-
Source
Chemical synthesis
-
Purity
> 90%
-
Application
Immunogen; SDS-PAGE; WB
-
Shipping Conditions
Ice packs
-
Storage conditions
Short term: -20°C; Long term: -80°C. Minimize freeze and thaw cycles.
-
Shelf life
1 year
-
Restriction
For research use only. Not for diagnostic procedures.
-
Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
-
Conjugation
Flag
-
Gene
Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.
Similar products
