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Category
Other Reagents
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Long description
The PIG3 ELISA is a sandwich enzyme immunoassay employing mouse monoclonal antibodies. A monoclonal antibody, specific for the human PIG3 protein, has been immobilized onto the surface of microtiter wells provided in the kit. The sample to be assayed and the biotinylated detector monoclonal antibody are pipetted into the wells and allowed to incubate for two hours, during which time any PIG3 present binds to the capture and detecting antibodies. Unbound material is washed away and horseradish peroxidase-conjugated streptavidin is added, which binds to the detector antibody._x000B_The horseradish peroxidase catalyzes the conversion of the chromogenic substrate tetra-methylbenzidine (TMB) from a colorless solution to a blue solution (or yellow after the addition of stop reagent), the intensity of which is proportional to the amount of PIG3 protein in the sample. The colored reaction product is quantified using a spectrophotometer._x000B_Quantitation is achieved by the construction of a standard curve using known concentrations_x000B_of PIG3 (provided lyophilized). By comparing the absorbance obtained from a sample containing an unknown amount of PIG3 with that obtained from the standards, the concentration of PIG3 in the sample can be determined.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from mice immunized with immunoprecipitated protein from phosphocellulose column flow through of extract of human breast cancer MCF-7 cells and mouse myeloma Sp2/0-Ag14 cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
PIG3 (p53 inducible gene 3) - ELISA Kit
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Antibody is raised in
see techfile
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
ELISA
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Antibody s suited for
This antibody can be used for gel supershift assays, flow cytometry, immunoprecipitation (2 µg/mg of protein lysate), Western blotting (1 µg/ml) or immunohistochemistry on frozen or formalin/paraffin fixed tissues (2-4 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Herrmann C, et al: Major vault protein of electric ray is a phosphoprotein. Neurochem Res 1998 Jan;23(1):39-46._x000B_2. Hamill DR, Suprenant KA: Characterization of the sea urchin major vault protein: a possible role for vault ribonucleoprotein particles in nucleocytoplasmic transport. Dev Biol 1997;190(1):117-128._x000B_3. Izquierdo MA, et al: Broad distribution of the multidrug resistance-related vault lung resistance protein in normal human tissues and tumors. Am J Pathol 1996 Mar;148(3):877-887._x000B_4. Hart SM, et al.: Expression of the human major vault protein LRP in acute myeloid leukemia. Exp Hematol 1997 Nov;25(12):1227-1232. _x000B_5. den Boer ML, et al: Relationship between major vault protein/lung resistance protein, multidrug resistance-associated protein, P-glycoprotein expression, and drug resistance in childhood leukemia. Blood 1998 Mar 15;91(6):2092-2098._x000B_6. Izquierdo MA, et al: Major vault protein LRP-related multidrug resistance. Eur J Cancer 1996 Jun;32A(6):979-984. _x000B_7. Schroeijers AB, et al.: Immunohistochemical detection of the human major vault protein LRP with two monoclonal antibodies in formalin-fixed, paraffin-embedded tissues. Am J Pathol 1998 Feb;152(2):373-378._x000B_8. Abbondanza C, et al: Interaction of vault particles with estrogen receptor in the MCF-7 breast cancer cell. J Cell Biol 1998;141(6):1301-1310._x000B_9. Dingemans AM, et al: Expression of the human major vault protein LRP in human lung cancer samples and normal lung tissues. Ann Oncol 1996 Aug;7(6):625-630. _x000B_10. Den Boer, M.L. et al. Relatiomship between major vault protein/lung resistance protein, multidrug resistance-associated protein, P-glycoprotein expression, and drug resistance in childhood leukemia. Blood 91, 2092-2098 (1998)._x000B_
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Description
cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
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Gene target
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Gene symbol
TP53I3, MRPL41
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Short name
PIG3 (p53 3) - ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Isotype
not specified
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Label
unlabeled
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Alternative name
PIG3 (p53 inducible gene 3) - Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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