FITC-xtra

  • Catalog number
    135
  • Price
    Please ask
  • Size
    5 mg
  • Description
    FITC-xtra is a fluorescent labeling dye used for preparing green fluorescent bioconjugates. There are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
  • Shipping
    Room Temperature
  • Storage
    Freezer
  • Shelflife
    12 months
  • Molecular Weight KD
    620.52
  • Appearance
    Solid
  • Solubility
    Soluble In: DMSO
  • Excitation Wavelength
    496 nm
  • Emission Wavelength
    524 nm
  • Personal precautions
    Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
  • Uses
    Laboratory chemicals *For Research Use Only*
  • Ordering
    To order FITC-xtra, please use the Cat. Nr.135 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for FITC-xtra
  • Properties
    This aat Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aat FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Gene target
    xtra  
  • Short name
    FITC-xtra
  • Technique
    FITC, Fluorescein
  • Label
    FITC
  • Alternative name
    fluorecein-xtra
  • Alternative technique
    fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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