FITC-xtra
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Catalog number135
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PricePlease ask
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Size5 mg
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DescriptionFITC-xtra is a fluorescent labeling dye used for preparing green fluorescent bioconjugates. There are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
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ShippingRoom Temperature
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StorageFreezer
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Shelflife12 months
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Molecular Weight KD620.52
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AppearanceSolid
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SolubilitySoluble In: DMSO
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Excitation Wavelength496 nm
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Emission Wavelength524 nm
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Personal precautionsWear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
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UsesLaboratory chemicals *For Research Use Only*
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OrderingTo order FITC-xtra, please use the Cat. Nr.135 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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TipsOur specialists recommend you to follow carefully the pre-registered instructions for FITC-xtra
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PropertiesThis aat Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aat FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Short nameFITC-xtra
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TechniqueFITC, Fluorescein
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LabelFITC
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Alternative namefluorecein-xtra
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Alternative techniquefluorescine
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data