FITC-dextran, 40 KDa

Shipping

The Shipping of FITC-dextran, 40 KDa is in gel packs
Storage

The storage of FITC-dextran, 40 KDa must be at - 4 C
Datasheet
Protocol

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Category

Protein
Concentration

25 mg/mL
Stability

The shelf life of FITC-dextran, 40 KDa is a maximum one year
Properties

This Chondrex Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Chondrex FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
Description

The kilo Daltons subunit weight of FITC-dextran, 40 compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein

Name

Fluorescent Antibody Technique
Concept

Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
Qualifiers

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